PMID- 18774660 OWN - NLM STAT- MEDLINE DCOM- 20090407 LR - 20090109 IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 133 IP - 4 DP - 2009 Feb 2 TI - First isolation of Histophilus somni from goats. PG - 383-6 LID - 10.1016/j.vetmic.2008.07.010 [doi] AB - Histophilus somni (former name: Haemophilus somnus) is a Gram-negative, facultative pathogen bacterium that colonises the mucous membranes of cattle and sheep, however it was also described in American bison and bighorn sheep. It can cause local or generalised diseases and asymptomatic carriers can also occur. The presence and the etiological role of this microorganism have not been confirmed in any other domesticated species yet. The purpose of this study was to prove the presence of H. somni in goats by bacterial isolation. Nasal, vaginal or praeputial swab samples were collected from 205 goats in 10 flocks. H. somni strains were isolated from 2 out of 10 flocks; in one flock 10 H. somni strains were isolated from the genital mucosa of 17 goats, while a single H. somni strain was cultured from a vagina of 26 animals in the other flock. Partial amplification and sequencing of the 16S rRNA gene of three H. somni strains verified the identification. The comparative examination of carbon source metabolism using the Biolog Microstation ID System (Biolog, Ca) showed a close relationship of the caprine strains, while they were less related to H. somni type strain CCUG-36157 of bovine origin. H. somni strains were isolated only in the oestrus season from goat flocks with sheep contact. This is the first paper on isolation of H. somni from goats. FAU - Janosi, Katalin AU - Janosi K AD - Department of Microbiology and Infectious Diseases, Szent Istvan University, 23-25 Hungaria krt., H-1143 Budapest, Hungary. Janosi.Katalin@aotk.szie.hu FAU - Hajtos, Istvan AU - Hajtos I FAU - Makrai, Laszlo AU - Makrai L FAU - Gyuranecz, Miklos AU - Gyuranecz M FAU - Varga, Janos AU - Varga J FAU - Fodor, Laszlo AU - Fodor L LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080726 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 SB - IM MH - Animals MH - Female MH - Genitalia, Male/microbiology MH - Goats/*microbiology MH - Male MH - Nose/microbiology MH - Pasteurellaceae/*isolation & purification MH - Vagina/microbiology EDAT- 2008/09/09 09:00 MHDA- 2009/04/08 09:00 CRDT- 2008/09/09 09:00 PHST- 2008/05/14 00:00 [received] PHST- 2008/07/15 00:00 [revised] PHST- 2008/07/17 00:00 [accepted] PHST- 2008/09/09 09:00 [pubmed] PHST- 2009/04/08 09:00 [medline] PHST- 2008/09/09 09:00 [entrez] AID - S0378-1135(08)00289-7 [pii] AID - 10.1016/j.vetmic.2008.07.010 [doi] PST - ppublish SO - Vet Microbiol. 2009 Feb 2;133(4):383-6. doi: 10.1016/j.vetmic.2008.07.010. Epub 2008 Jul 26.