PMID- 18775856
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20221207
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 50
IP  - 2
DP  - 2009 Feb
TI  - Tight glycemic control regulates fibronectin expression and basement membrane 
      thickening in retinal and glomerular capillaries of diabetic rats.
PG  - 943-9
LID - 10.1167/iovs.08-2377 [doi]
AB  - PURPOSE: To determine whether tight glycemic control prevents development of 
      basement membrane (BM) thickening in retinal and glomerular capillaries of 
      diabetic rats and whether the extent to which BM thickening develops is linked to 
      fibronectin (FN) overexpression and the degree of hyperglycemia. METHODS: Retinal 
      and renal cortical tissues obtained from the tightly controlled diabetic (TC), 
      poorly controlled diabetic (D), and nondiabetic (N) control rats were subjected 
      to morphometric and biochemical analyses. In both tissues, capillary BM 
      thickening was determined by electron microscopy, and FN protein level was 
      assessed by Western blot analysis. Routine measurements of blood glucose level 
      and glycohemoglobin level were performed throughout the study. RESULTS: The HbA1c 
      level was significantly increased in D rats, but not in TC rats, compared with 
      those of the N rats with a concomitant increase in capillary BM thickness and FN 
      protein expression in retinal and renal tissues. A strong correlation was 
      observed between retinal and glomerular capillary BM thickness (r=0.79, 
      P=0.0001), between retinal and kidney FN protein levels (r=0.7, P=0.005), between 
      HbA1c and FN protein levels in the retina (r=0.66, P=0.006) and kidney (r= .84, 
      P=0.0003), and between HbA1c level and BM thickness in retinal (r=0.76, P=0.0002) 
      and renal tissues (r=0.64, P=0.004). CONCLUSIONS: In diabetes BM thickening 
      develops in retinal and glomerular capillaries in a correlated manner. Tight 
      glycemic control may be beneficial in preventing the pathologic development of 
      capillary BM thickening and FN overexpression in retinal and renal tissues, two 
      target tissues of diabetic microangiopathy.
FAU - Cherian, Saira
AU  - Cherian S
AD  - Department of Medicine, Boston University School of Medicine, Boston, 
      Massachusetts 02118, USA.
FAU - Roy, Sumon
AU  - Roy S
FAU - Pinheiro, Andre
AU  - Pinheiro A
FAU - Roy, Sayon
AU  - Roy S
LA  - eng
GR  - EY14702/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080904
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Blood Glucose)
RN  - 0 (Fibronectins)
RN  - 0 (Glycated Hemoglobin A)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Basement Membrane/*ultrastructure
MH  - Blood Glucose/analysis
MH  - Blotting, Western
MH  - Capillaries/metabolism/pathology
MH  - Diabetes Mellitus, Experimental/metabolism/pathology
MH  - Diabetic Nephropathies/*metabolism/pathology
MH  - Diabetic Retinopathy/*metabolism/pathology
MH  - Fibronectins/*metabolism
MH  - Glucose/metabolism
MH  - Glycated Hemoglobin/analysis
MH  - Hyperglycemia/*metabolism
MH  - Kidney Glomerulus/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinal Vessels/*metabolism/*ultrastructure
EDAT- 2008/09/09 09:00
MHDA- 2009/02/07 09:00
CRDT- 2008/09/09 09:00
PHST- 2008/09/09 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
PHST- 2008/09/09 09:00 [entrez]
AID - iovs.08-2377 [pii]
AID - 10.1167/iovs.08-2377 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2009 Feb;50(2):943-9. doi: 10.1167/iovs.08-2377. Epub 
      2008 Sep 4.