PMID- 18776035
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 74
IP  - 20
DP  - 2008 Oct
TI  - In situ detection of freshwater fungi in an alpine stream by new taxon-specific 
      fluorescence in situ hybridization probes.
PG  - 6427-36
LID - 10.1128/AEM.00815-08 [doi]
AB  - New rRNA-targeting oligonucleotide probes permitted the fluorescence in situ 
      hybridization (FISH) identification of freshwater fungi in an Austrian 
      second-order alpine stream. Based on computer-assisted comparative sequence 
      analysis, nine taxon-specific probes were designed and evaluated by whole-fungus 
      hybridizations. Oligonucleotide probe MY1574, specific for a wide range of 
      Eumycota, and the genus (Tetracladium)-specific probe TCLAD1395, as well as the 
      species-specific probes ALacumi1698 (Alatospora acuminata), TRIang322 (Tricladium 
      angulatum), and Alongi340 (Anguillospora longissima), are targeted against 18S 
      rRNA, whereas probes TmarchB10, TmarchC1_1, TmarchC1_2, and AlongiB16 are 
      targeted against the 28S rRNA of Tetracladium marchalianum and Anguillospora 
      longissima, respectively. After 2 weeks and 3 months of exposure of polyethylene 
      slides in the stream, attached germinating conidia and growing hyphae of 
      freshwater fungi were accessible for FISH. Growing hyphae and germinating conidia 
      on leaves and in membrane cages were also visualized by the new FISH probes.
FAU - Baschien, Christiane
AU  - Baschien C
AD  - Department of Environmental Microbiology, Berlin Institute of Technology, Berlin, 
      Germany. christiane.baschien@tu-berlin.de
FAU - Manz, Werner
AU  - Manz W
FAU - Neu, Thomas R
AU  - Neu TR
FAU - Marvanova, Ludmila
AU  - Marvanova L
FAU - Szewzyk, Ulrich
AU  - Szewzyk U
LA  - eng
SI  - GENBANK/AY204573
SI  - GENBANK/AY204574
SI  - GENBANK/AY204575
SI  - GENBANK/AY204576
SI  - GENBANK/AY204577
SI  - GENBANK/AY204578
SI  - GENBANK/AY204583
SI  - GENBANK/AY204584
SI  - GENBANK/AY204585
SI  - GENBANK/AY204586
SI  - GENBANK/AY204597
SI  - GENBANK/AY204599
SI  - GENBANK/AY204600
SI  - GENBANK/AY204601
SI  - GENBANK/AY204602
SI  - GENBANK/AY204603
SI  - GENBANK/AY204604
SI  - GENBANK/AY204605
SI  - GENBANK/AY204606
SI  - GENBANK/AY204607
SI  - GENBANK/AY204612
SI  - GENBANK/AY204613
SI  - GENBANK/AY204614
SI  - GENBANK/AY204615
SI  - GENBANK/AY204616
SI  - GENBANK/AY204617
SI  - GENBANK/AY204618
SI  - GENBANK/AY204619
SI  - GENBANK/AY204626
SI  - GENBANK/AY204627
SI  - GENBANK/AY204628
SI  - GENBANK/AY204629
SI  - GENBANK/AY204630
SI  - GENBANK/AY204631
SI  - GENBANK/AY204632
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080905
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Fungal)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 28S)
SB  - IM
MH  - Austria
MH  - DNA, Fungal/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Fresh Water/*microbiology
MH  - Fungi/genetics/*isolation & purification
MH  - Hyphae/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes/*genetics
MH  - RNA, Fungal/genetics
MH  - RNA, Ribosomal, 18S/genetics
MH  - RNA, Ribosomal, 28S/genetics
MH  - Rivers
MH  - Sequence Analysis, DNA
MH  - Spores, Fungal/genetics
PMC - PMC2570288
EDAT- 2008/09/09 09:00
MHDA- 2008/11/19 09:00
PMCR- 2009/04/01
CRDT- 2008/09/09 09:00
PHST- 2008/09/09 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/09/09 09:00 [entrez]
PHST- 2009/04/01 00:00 [pmc-release]
AID - AEM.00815-08 [pii]
AID - 0815-08 [pii]
AID - 10.1128/AEM.00815-08 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2008 Oct;74(20):6427-36. doi: 10.1128/AEM.00815-08. Epub 
      2008 Sep 5.