PMID- 187762
OWN - NLM
STAT- MEDLINE
DCOM- 19770216
LR  - 20190509
IS  - 0027-8874 (Print)
IS  - 0027-8874 (Linking)
VI  - 57
IP  - 1
DP  - 1976 Jul
TI  - Nucleic acids from subcellular fractions of N-nitrosodiethylamine-induced 
      hepatoma in mice.
PG  - 23-31
AB  - During eight successive isologous passages of hepatoma induced in male C3HA mice 
      by N-nitrosodiethylamine, no common features of tumor progression were observed, 
      although both the mitotic pattern and ploidy differed from generation to 
      generation. These additional cytologic criteria allowed the biochemical 
      examination of material least changed due to tumor progression. Tumor nDNA's were 
      characterized by greater actinomycin D (AD)- and acridine orange (AO)-binding 
      abilities than were normal nDNA's; this could have resulted from a higher 
      proportion of double-stranded regions in tumor DNA. Isolated tumor 
      deoxyribonucleoprotein had both lower template activity in an RNA polymerase 
      system and fewer AD- and AO-binding sites, when compared with the activity and 
      sites from normal mouse liver. RNA-DNA hybridization data with the 
      above-mentioned findings showed that in hepatoma, part of the nuclear genome was 
      repressed. Also, RNA "new classes" appeared and a certain proportion of nuclear 
      genes controlling mitochondrial protein biosynthesis were derepressed in tumor 
      mitochondria. The hybridization of mitochondrial RNA (mtRNA) and DNA revealed new 
      classes of pulse-labeled RNA's in in vitro-incubated liver mitochondria that were 
      absent from intact cell organelles; the hybridization properties of in vivo- and 
      in vitro-formed hepatoma mtRNA's were similar. Competition and hybridization 
      experiments demonstrated that in tumor mitochondria in vivo, some new classes of 
      RNA existed. Hepatoma mitochondrial mRNA had a higher metabolic stability than 
      did normal mRNA.
FAU - Shaposhnikov, J D
AU  - Shaposhnikov JD
FAU - Shalumovich, W N
AU  - Shalumovich WN
FAU - Kisselev, O I
AU  - Kisselev OI
FAU - Gaitskhoki, V S
AU  - Gaitskhoki VS
FAU - Pozharisski, K M
AU  - Pozharisski KM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Natl Cancer Inst
JT  - Journal of the National Cancer Institute
JID - 7503089
RN  - 0 (Acridines)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 3IQ78TTX1A (Diethylnitrosamine)
SB  - IM
MH  - Acridines/metabolism
MH  - Animals
MH  - Carcinoma, Hepatocellular/chemically induced/*metabolism/pathology
MH  - Cell Nucleus/metabolism
MH  - DNA, Neoplasm/*metabolism
MH  - Dactinomycin/metabolism
MH  - Diethylnitrosamine
MH  - Genes
MH  - Liver Neoplasms/chemically induced/*metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mitochondria, Liver/metabolism
MH  - Mitosis
MH  - Neoplasms, Experimental/chemically induced/metabolism/pathology
MH  - RNA, Messenger/metabolism
MH  - RNA, Neoplasm/*metabolism
EDAT- 1976/07/01 00:00
MHDA- 1976/07/01 00:01
CRDT- 1976/07/01 00:00
PHST- 1976/07/01 00:00 [pubmed]
PHST- 1976/07/01 00:01 [medline]
PHST- 1976/07/01 00:00 [entrez]
AID - 10.1093/jnci/57.1.23 [doi]
PST - ppublish
SO  - J Natl Cancer Inst. 1976 Jul;57(1):23-31. doi: 10.1093/jnci/57.1.23.