PMID- 18776639
OWN - NLM
STAT- MEDLINE
DCOM- 20081028
LR  - 20211020
IS  - 0022-1333 (Print)
IS  - 0022-1333 (Linking)
VI  - 87
IP  - 2
DP  - 2008 Aug
TI  - Chromosomal organization of simple sequence repeats in the Pacific oyster 
      (Crassostrea gigas): (GGAT)(4), (GT)(7) and (TA)(10) chromosome patterns.
PG  - 119-25
AB  - Chromosome identification is essential in oyster genomic research. Fluorescence 
      in situ hybridization (FISH) offers new opportunities for the identification of 
      oyster chromosomes. It has been used to locate satellite DNAs, telomeres or 
      ribosomal DNA sequences. However, regarding chromosome identification, no study 
      has been conducted with simple sequence repeats (SSRs). FISH was used to probe 
      the physical organization of three particular SSRs, (GGAT)(4), (GT)(7) and 
      (TA)(10) onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. 
      Hybridization signals were observed in all the SSR probes, but the distribution 
      and intensity of signals varied according to the oligonucleotide repeat. The 
      intercalary, centromeric and telomeric bands were observed along the chromosomes, 
      and for each particular repeat every chromosome pair presented a similar pattern, 
      allowing karyotypic analysis with all the SSRs tested. Our study is the first in 
      mollusks to show the application of SSR in situ hybridization for chromosome 
      identification and karyotyping. This technique can be a useful tool for oyster 
      comparative studies and to understand genome organization in different oyster 
      taxa.
FAU - Bouilly, K
AU  - Bouilly K
AD  - Institute for Biotechnology and Bioengineering, Centre of Genetics and 
      Biotechnology, University of Tras-os-Montes and Alto Douro, (IBB/CGB-UTAD), 
      5001-801 Vila Real, Portugal. kbouilly@yahoo.fr
FAU - Chaves, R
AU  - Chaves R
FAU - Leitao, A
AU  - Leitao A
FAU - Benabdelmouna, A
AU  - Benabdelmouna A
FAU - Guedes-Pinto, H
AU  - Guedes-Pinto H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - J Genet
JT  - Journal of genetics
JID - 2985113R
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Banding
MH  - Chromosomes/*genetics
MH  - Crassostrea/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Minisatellite Repeats/*genetics
EDAT- 2008/09/09 09:00
MHDA- 2008/10/29 09:00
CRDT- 2008/09/09 09:00
PHST- 2008/09/09 09:00 [pubmed]
PHST- 2008/10/29 09:00 [medline]
PHST- 2008/09/09 09:00 [entrez]
AID - 10.1007/s12041-008-0018-2 [doi]
PST - ppublish
SO  - J Genet. 2008 Aug;87(2):119-25. doi: 10.1007/s12041-008-0018-2.