PMID- 18777114
OWN - NLM
STAT- MEDLINE
DCOM- 20090113
LR  - 20101118
IS  - 1023-3830 (Print)
IS  - 1023-3830 (Linking)
VI  - 57
IP  - 9
DP  - 2008 Sep
TI  - Signal pathway involved in inhibition by lipoxin A(4) of production of 
      interleukins induced in endothelial cells by lipopolysaccharide.
PG  - 430-7
LID - 10.1007/s00011-008-7147-1 [doi]
AB  - OBJECTIVE AND DESIGN: We examine whether lipoxin A(4) (LXA(4)) inhibits 
      production of interleukins (ILs) in endothelial cells and what signal pathway 
      might participate in the actions of LXA(4). METHODS: Cultured pulmonary 
      microvascular endothelial cells (PMVEC) were treated with lipopolysaccharide 
      (LPS), with or without preincubation with LXA(4). RESULTS: The results showed 
      that LPS induced production of IL-1beta, IL-6 and IL-8 in rat PMVEC, upregulated 
      the expressions of myeloid differentiation factor 88 (MyD88), phosphorylated p38 
      and p42/44 mitogen-activated protein kinase (MAPK), phosphorylated 
      phosphoinositide 3-kinase (PI3-K), DNA-binding activities of nuclear factor-kappa 
      B (NF-kappaB) and activator protein-1(AP-1). The blockade of p38 MAPK, p42/44 
      MAPK, PI3-K, NF-kappaB or AP-1 partially inhibited production of IL-1beta, IL-6 
      and IL-8 stimulated by LPS, respectively. LXA(4) significantly inhibited 
      LPS-stimulated secretion of protein and expressions of mRNA of IL-1beta, IL-6 and 
      IL-8, activation of p38 MAPK, p42/44 MAPK, PI3-K, NF-kappaB and AP-1 but not 
      MyD88 in PMVEC. CONCLUSIONS: LXA(4) inhibits synthesis of IL-1beta, IL-6 and IL-8 
      in PMVEC and this antagonism is related to PI3-K, p38 and p42/44 MAPK, NF-kappaB 
      and AP-1 pathway-dependent signal transduction.
FAU - Wu, Sheng-Hua
AU  - Wu SH
AD  - Department of Pediatrics, Central Laboratory, the First Affiliated Hospital of 
      Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, People's 
      Republic of China.
FAU - Liao, P-Y
AU  - Liao PY
FAU - Dong, L
AU  - Dong L
FAU - Chen, Z-Q
AU  - Chen ZQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Interleukins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Lipoxins)
RN  - 0 (Myd88 protein, rat)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (lipoxin A4)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Endothelial Cells/*drug effects/immunology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Inflammation/drug therapy
MH  - Interleukins/*biosynthesis
MH  - Lipopolysaccharides/*pharmacology
MH  - Lipoxins/*pharmacology
MH  - Male
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - NF-kappa B/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - *Signal Transduction
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2008/09/09 09:00
MHDA- 2009/01/14 09:00
CRDT- 2008/09/09 09:00
PHST- 2008/09/09 09:00 [pubmed]
PHST- 2009/01/14 09:00 [medline]
PHST- 2008/09/09 09:00 [entrez]
AID - 10.1007/s00011-008-7147-1 [doi]
PST - ppublish
SO  - Inflamm Res. 2008 Sep;57(9):430-7. doi: 10.1007/s00011-008-7147-1.