PMID- 18778287
OWN - NLM
STAT- MEDLINE
DCOM- 20090617
LR  - 20211020
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 127
IP  - 1
DP  - 2009 May
TI  - Expansion of CD4+ CD25+ Foxp3+ T cells by bone marrow-derived dendritic cells.
PG  - 50-61
LID - 10.1111/j.1365-2567.2008.02927.x [doi]
AB  - Dendritic cells (DCs) are the most important antigen-presenting cells of the 
      immune system and have a crucial role in T-lymphocyte activation and adaptive 
      immunity initiation. However, DCs have also been implicated in maintaining 
      immunological tolerance. In this study, we evaluated changes in the CD4(+) 
      CD25(+) Foxp3(+) T-cell population after co-culture of lymph node cells from 
      BALB/c mice with syngeneic bone marrow-derived DCs. Our results showed an 
      increase in CD4(+) CD25(+) Foxp3(+) T cells after co-culture which occurred 
      regardless of the activation state of DCs and the presence of allogeneic 
      apoptotic cells; however, it was greater when DCs were immature and were pulsed 
      with the alloantigen. Interestingly, syngeneic apoptotic thymocytes were not as 
      efficient as allogeneic apoptotic cells in expanding the CD4(+) CD25(+) Foxp3(+) 
      T-cell population. In all experimental settings, DCs produced high amounts of 
      transforming growth factor (TGF)-beta. The presence of allogeneic apoptotic cells 
      induced interleukin (IL)-2 production in immature and mature DC cultures. This 
      cytokine was also detected in the supernatants under all experimental conditions 
      and enhanced when immature DCs were pulsed with the alloantigen. CD4(+) CD25(+) 
      Foxp3(+) T-cell expansion during co-culture of lymph node cells with DCs strongly 
      suggested that the presence of alloantigen enhanced the number of regulatory T 
      cells (Tregs) in vitro. Our data also suggest a role for both TGF-beta and IL-2 
      in the augmentation of the CD4(+) CD25(+) Foxp3(+) population.
FAU - Marguti, Ivo
AU  - Marguti I
AD  - Laboratory of Clinical Immunology, Department of Immunology, Instituto de 
      Ciencias Biomedicas, University of Sao Paulo, Sao Paulo, Brazil.
FAU - Yamamoto, Guilherme Lopes
AU  - Yamamoto GL
FAU - da Costa, Thais Boccia
AU  - da Costa TB
FAU - Rizzo, Luiz Vicente
AU  - Rizzo LV
FAU - de Moraes, Luciana Vieira
AU  - de Moraes LV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Interleukin-2)
SB  - IM
MH  - Animals
MH  - Apoptosis/immunology
MH  - Cell Differentiation/immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Forkhead Transcription Factors/*analysis
MH  - Immunophenotyping
MH  - Interleukin-2/biosynthesis
MH  - Lymph Nodes/immunology
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Phagocytosis/immunology
MH  - T-Lymphocytes, Regulatory/*immunology
PMC - PMC2678181
EDAT- 2008/09/10 09:00
MHDA- 2009/06/18 09:00
PMCR- 2010/05/01
CRDT- 2008/09/10 09:00
PHST- 2008/09/10 09:00 [pubmed]
PHST- 2009/06/18 09:00 [medline]
PHST- 2008/09/10 09:00 [entrez]
PHST- 2010/05/01 00:00 [pmc-release]
AID - IMM2927 [pii]
AID - 10.1111/j.1365-2567.2008.02927.x [doi]
PST - ppublish
SO  - Immunology. 2009 May;127(1):50-61. doi: 10.1111/j.1365-2567.2008.02927.x.