PMID- 1877884 OWN - NLM STAT- MEDLINE DCOM- 19910925 LR - 20190815 IS - 0304-8608 (Print) IS - 0304-8608 (Linking) VI - 119 IP - 3-4 DP - 1991 TI - The free nucleocapsids of the viral haemorrhagic septicaemia virus contain two antigenically related nucleoproteins. PG - 153-63 AB - A protein of 34 kDa (Nx) was induced in vitro by the infection of fish cell cultures with the rhabdovirus agent of viral haemorrhagic septicaemia (VHS) of the trout. This protein only appeared as a major component in concentrated or intracellular labeled VHS virus but not in purified VHS or in the related infectious haematopoietic necrosis virus. That Nx protein is antigenically related to the nucleoprotein of purified virus was shown by its reaction with four anti-nucleoprotein monoclonal antibodies (at least 3 of them reacting non competitively against different epitopes) and by immunoprecipitation with polyvalent international reference sera. The Nx protein was shown to be specifically associated with free non-infective particles isolated by ultracentrifugation which were confirmed to be nucleocapsids by electron microscopy. FAU - Basurco, B AU - Basurco B AD - Centro de Investigacion y Tecnologia del I.N.I.A., Madrid, Spain. FAU - Sanz, F AU - Sanz F FAU - Marcotegui, M A AU - Marcotegui MA FAU - Coll, J M AU - Coll JM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 RN - 0 (Antibodies, Monoclonal) RN - 0 (Viral Core Proteins) SB - IM MH - Animals MH - Antibodies, Monoclonal MH - Capsid/*analysis/immunology MH - Cells, Cultured MH - Fishes/microbiology MH - Immunoblotting MH - Rhabdoviridae/*analysis/immunology/isolation & purification/ultrastructure MH - Viral Core Proteins/*analysis/immunology EDAT- 1991/01/01 00:00 MHDA- 1991/01/01 00:01 CRDT- 1991/01/01 00:00 PHST- 1991/01/01 00:00 [pubmed] PHST- 1991/01/01 00:01 [medline] PHST- 1991/01/01 00:00 [entrez] AID - 10.1007/BF01310666 [doi] PST - ppublish SO - Arch Virol. 1991;119(3-4):153-63. doi: 10.1007/BF01310666.