PMID- 18779337
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20211020
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 76
IP  - 11
DP  - 2008 Nov
TI  - Recruitment of Rab27a to phagosomes controls microbial antigen cross-presentation 
      by dendritic cells.
PG  - 5373-80
LID - 10.1128/IAI.01044-08 [doi]
AB  - Polyreactive immunoglobulins (Ig) and complement components are present in 
      tissues and blood of healthy individuals. They facilitate pathogen uptake and 
      inactivation in lysosomes of phagocytes and thereby provide rapid protection 
      against infection. Dendritic cells (DCs) are phagocytes that can acquire peptides 
      from phagocytosed antigen to elicit cytotoxic immune responses by CD8(+) T 
      lymphocytes. The mechanisms that select peptides for cross-presentation are not 
      fully resolved. Here we investigated the role of polyreactive Ig and complement 
      in directing phagosomal antigen processing for cross-presentation. Phagocytosis 
      facilitated by serum opsonization required the presence of Ig for effective 
      antigen cross-presentation of microbe-derived antigen. The presence of complement 
      C3 in serum promoted phagocytosis, yet phagosomes were defective in antigen 
      degradation. The small GTPase Rab27a was recently implicated in antigen 
      cross-presentation and was rapidly recruited to phagosomes only when Ig was 
      present. Our data suggest that prebinding of antigen by polyreactive Ig 
      potentiates the efficiency of antigen cross-presentation to CD8(+) T cells 
      through recruitment of Rab27a.
FAU - Kim, Seong Hyun
AU  - Kim SH
AD  - Department of Dermatology, Brigham and Women's Hospital and Harvard Medical 
      School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
FAU - Visser, Annelies
AU  - Visser A
FAU - Cruijsen, Carin
AU  - Cruijsen C
FAU - van der Velden, Adrianus W M
AU  - van der Velden AW
FAU - Boes, Marianne
AU  - Boes M
LA  - eng
GR  - R01 AR052810/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080908
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Complement C3)
RN  - 0 (Immunoglobulins)
RN  - 0 (rab27 GTP-Binding Proteins)
RN  - EC 3.6.1.-. (Rab27a protein, mouse)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigens, Bacterial/*immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Complement C3/immunology
MH  - Cross-Priming/*immunology
MH  - Dendritic Cells/*immunology
MH  - Escherichia coli/immunology
MH  - Flow Cytometry
MH  - Immunoglobulins/immunology
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Microscopy, Confocal
MH  - Phagocytosis/immunology
MH  - Phagosomes/*metabolism
MH  - rab GTP-Binding Proteins/*metabolism
MH  - rab27 GTP-Binding Proteins
PMC - PMC2573350
EDAT- 2008/09/10 09:00
MHDA- 2008/11/19 09:00
PMCR- 2009/05/01
CRDT- 2008/09/10 09:00
PHST- 2008/09/10 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/09/10 09:00 [entrez]
PHST- 2009/05/01 00:00 [pmc-release]
AID - IAI.01044-08 [pii]
AID - 1044-08 [pii]
AID - 10.1128/IAI.01044-08 [doi]
PST - ppublish
SO  - Infect Immun. 2008 Nov;76(11):5373-80. doi: 10.1128/IAI.01044-08. Epub 2008 Sep 
      8.