PMID- 18781392 OWN - NLM STAT- MEDLINE DCOM- 20091005 LR - 20211020 IS - 1573-5168 (Electronic) IS - 0920-1742 (Linking) VI - 35 IP - 3 DP - 2009 Aug TI - Morphological and histochemical characteristics of the epithelium of ovarian lamellae of Genypterus blacodes (Schneider, 1801). PG - 359-67 LID - 10.1007/s10695-008-9256-8 [doi] AB - The physiological significance of the glycoproteins (GPs) secreted by the epithelium of ovarian lamellae is discussed in reference to the reproductive biology of G. blacodes. Histochemical procedures for localising and characterising GPs were used to determine the cytoplasmic components of cells of the epithelium that covers the ovarian lamellae of pink cuskeel, Genypterus blacodes (Schneider, 1801) (Pisces, Ophidiidae), during spawning. This species is one of the most valuable demersal fish resources in the Argentine Sea, mainly due its large size and flesh quality. GPs with oxidizable vicinal diol groups, sialic acid with or without O-acyl substituents, O-acyl sugars, neutral sugars and GPs with carboxyl and sulphate groups were detected. Light microscope examination showed morphological changes in the epithelium of ovarian lamellae during the spawning season, associated with a secretory activity of mucus. Optical density studies revealed the presence of polyploid cells encompassing those morphological changes. Results of the present study suggest that the epithelium of ovarian lamellae of G. blacodes performs a secretory role, which is intensified during ovarian maturity, suggesting that G. blacodes could release masses of eggs enveloped in mucus. FAU - Freijo, R O AU - Freijo RO AD - Departamento de Biologia, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, 3 piso, 7600, Mar del Plata, Argentina. FAU - Garcia, A M AU - Garcia AM FAU - Portiansky, E L AU - Portiansky EL FAU - Barbeito, C G AU - Barbeito CG FAU - Macchi, G J AU - Macchi GJ FAU - Diaz, A O AU - Diaz AO LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080910 PL - Netherlands TA - Fish Physiol Biochem JT - Fish physiology and biochemistry JID - 100955049 SB - IM MH - Animals MH - Argentina MH - Atlantic Ocean MH - Epithelium/*anatomy & histology MH - Female MH - Fishes/*anatomy & histology/metabolism MH - Histocytochemistry MH - Microscopy MH - Ovary/*anatomy & histology EDAT- 2008/09/11 09:00 MHDA- 2009/10/06 06:00 CRDT- 2008/09/11 09:00 PHST- 2008/02/28 00:00 [received] PHST- 2008/07/27 00:00 [accepted] PHST- 2008/09/11 09:00 [pubmed] PHST- 2009/10/06 06:00 [medline] PHST- 2008/09/11 09:00 [entrez] AID - 10.1007/s10695-008-9256-8 [doi] PST - ppublish SO - Fish Physiol Biochem. 2009 Aug;35(3):359-67. doi: 10.1007/s10695-008-9256-8. Epub 2008 Sep 10.