PMID- 18782116
OWN - NLM
STAT- MEDLINE
DCOM- 20081117
LR  - 20090521
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 63
IP  - 10
DP  - 2008 Oct
TI  - Staphylococcus aureus in nasal lavage and biopsy of patients with chronic 
      rhinosinusitis.
PG  - 1359-67
LID - 10.1111/j.1398-9995.2008.01798.x [doi]
AB  - BACKGROUND: Staphylococcus aureus may play a relevant etiologic role in chronic 
      rhinosinusitis (CRS) and may explain the T(H2) shift observed in CRS with nasal 
      polyps (CRSNP(+)). Naturally occurring S. aureus small colony variants (SASCV) 
      escape immune surveillance, antibiotic treatment and microbiologic routine 
      diagnostic techniques. The frequency of S. aureus and SASCV in CRS patients and 
      S. aureus-related effects on the local immune response should be prospectively 
      investigated. METHODS: Nasal lavages and mucosal biopsies of CRS patients were 
      examined with bacterial culture suitable for detecting SASCV, real time PCR and 
      fluorescence in situ hybridization. To assess the effects of S. aureus 
      positivity, interleukin-5 (IL-5), interferon-gamma, total immunoglobulin E (IgE), 
      eotaxin, granulocyte-colony stimulating factor, and eosinophil cationic protein 
      in nasal lavages were determined and gene transcription analysis of nasal 
      biopsies from S. aureus positive and negative CRSNP(+) patients was performed. 
      RESULTS: Thirty-one CRSNP(+) patients, 13 CRS patients without polyps, and 21 
      control patients were evaluated. Staphylococcus aureus was detected by any method 
      in 25 patients (39%). Staphylococcus aureus detection rates did not differ 
      between the three disease groups (P = 0.3). Staphylococcus aureus small colony 
      variants were not found. In nasal lavages, IL-5 and total IgE levels were higher 
      in CRSNP(+) patients than in CRSNP(-) patients or controls (P < 0.05). 
      Staphylococcus aureus positivity did not influence biomarker concentrations in 
      nasal lavages. Genes for T(H2) cytokines were not differentially transcribed. 
      CONCLUSIONS: We could not observe a higher prevalence of S. aureus in CRS 
      patients with or without nasal polyps than in controls. We could not substantiate 
      that S. aureus intensifies the T(H2) shift in CRSNP(+) patients. Staphylococcus 
      aureus small colony variants were not detected in any sample.
FAU - Niederfuhr, A
AU  - Niederfuhr A
AD  - Department of Otorhinolaryngology, University Hospital of Ulm, Ulm, Germany.
FAU - Kirsche, H
AU  - Kirsche H
FAU - Deutschle, T
AU  - Deutschle T
FAU - Poppert, S
AU  - Poppert S
FAU - Riechelmann, H
AU  - Riechelmann H
FAU - Wellinghausen, N
AU  - Wellinghausen N
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
SB  - IM
CIN - Allergy. 2009 Jun;64(6):959-60. PMID: 19416138
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - Chronic Disease
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nasal Lavage Fluid/immunology/*microbiology
MH  - Nasal Mucosa/immunology/microbiology/*pathology
MH  - Prospective Studies
MH  - Rhinitis/immunology/microbiology/*pathology
MH  - Sinusitis/immunology/microbiology/*pathology
MH  - Staphylococcal Infections/immunology/*pathology
MH  - Staphylococcus aureus/growth & development/immunology
EDAT- 2008/09/11 09:00
MHDA- 2008/11/18 09:00
CRDT- 2008/09/11 09:00
PHST- 2008/09/11 09:00 [pubmed]
PHST- 2008/11/18 09:00 [medline]
PHST- 2008/09/11 09:00 [entrez]
AID - ALL1798 [pii]
AID - 10.1111/j.1398-9995.2008.01798.x [doi]
PST - ppublish
SO  - Allergy. 2008 Oct;63(10):1359-67. doi: 10.1111/j.1398-9995.2008.01798.x.