PMID- 18782664
OWN - NLM
STAT- MEDLINE
DCOM- 20090212
LR  - 20090724
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 24
IP  - 4
DP  - 2008 Dec 1
TI  - Patterning of cells on functionalized poly(dimethylsiloxane) surface prepared by 
      hydrophobin and collagen modification.
PG  - 918-22
LID - 10.1016/j.bios.2008.07.045 [doi]
AB  - Controllable cell growth on poly(dimethylsiloxzne) (PDMS) surface is important 
      for its potential applications in biodevices. Herein, we developed a fully 
      biocompatible approach for patterning of cells on the PDMS surface by hydrophobin 
      (HFBI) and collagen modification. HFBI and collagen were immobilized on the PDMS 
      surface one after another by using copper grids as a mask. HFBI self-assembly on 
      PDMS surface converted the PDMS surface from hydrophobic to hydrophilic, which 
      facilitated the following immobilization of collagen. Collagen had admirable 
      ability to support cell adhesion and growth. Consequently, the 
      HFBI/collagen-modified PDMS surface could promote cell adhesion and growth. What 
      is more, the native PDMS surface did not support cell adhesion and growth. 
      Patterning of cells was achieved by directly culturing 293T cells (the human 
      embryonic kidney cell line) on the PDMS surface patterned with HFBI/collagen. 
      Further studies by means of gene transfection experiment in vitro showed that the 
      patterned cells were of good bioactivities. Herein, the biocompatible preparation 
      of cell patterns on the PDMS surface could be of many applications in biosensor 
      device fabrication.
FAU - Hou, Sen
AU  - Hou S
AD  - The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life 
      Science, Nankai University, Tianjin 300071, PR China.
FAU - Yang, Kun
AU  - Yang K
FAU - Qin, Ming
AU  - Qin M
FAU - Feng, Xi-Zeng
AU  - Feng XZ
FAU - Guan, Li
AU  - Guan L
FAU - Yang, Yanlian
AU  - Yang Y
FAU - Wang, Chen
AU  - Wang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080803
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Fungal Proteins)
RN  - 63148-62-9 (baysilon)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Biocompatible Materials/*chemistry
MH  - Cell Adhesion
MH  - Cell Culture Techniques/*instrumentation/*methods
MH  - Cell Line
MH  - Collagen/chemistry/*metabolism
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Fungal Proteins/chemistry/*metabolism
MH  - Humans
MH  - Kidney/*physiology
MH  - Protein Binding
MH  - Surface Properties
EDAT- 2008/09/11 09:00
MHDA- 2009/02/13 09:00
CRDT- 2008/09/11 09:00
PHST- 2008/05/12 00:00 [received]
PHST- 2008/07/01 00:00 [revised]
PHST- 2008/07/21 00:00 [accepted]
PHST- 2008/09/11 09:00 [pubmed]
PHST- 2009/02/13 09:00 [medline]
PHST- 2008/09/11 09:00 [entrez]
AID - S0956-5663(08)00403-X [pii]
AID - 10.1016/j.bios.2008.07.045 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2008 Dec 1;24(4):918-22. doi: 10.1016/j.bios.2008.07.045. 
      Epub 2008 Aug 3.