PMID- 18783137
OWN - NLM
STAT- MEDLINE
DCOM- 20081002
LR  - 20160510
IS  - 1210-7875 (Print)
IS  - 1210-7875 (Linking)
VI  - 44
IP  - 3
DP  - 2008 Jul
TI  - A comparision of RT-PCR and FISH techniques in molecular diagnosis of Ewing's 
      sarcoma in paraffin-embedded tissue.
PG  - 67-70
AB  - Ewing's sarcoma is relatively uncommon tumor representing 6-8 percent of 
      malignant bone tumors with variable morphology. Cytogenetically, Ewing's sarcomas 
      are characterized by a specific reciprocal chromosomal translocation 
      t(11;22)(q24;q12). The presence of this chromosomal translocation has been 
      detected in approximately 85 percent of the cases. The translocation results in 
      the fusion of EWS gene from chromosome 22 to FLI1 gene at 11q24 which is a member 
      of ETS family of transcription factors. In this study we performed a comparison 
      of two molecular diagnostic strategies, namely RT-PCR and FISH, in fresh, frozen 
      and formalin-fixed paraffin-embedded tissues. We conclude that FISH is a more 
      sensitive technique than RT-PCR for the diagnosis of Ewing's tumors in 
      formalin-fixed paraffin-embedded tissue. In conclusion, molecular pathology 
      techniques, using reverse transcription-polymerase chain reaction (RT-PCR) and/or 
      fluorescence in situ hybridization (FISH) are valuable diagnostic tools for 
      evaluation of undifferentiated small round-cell tumors like Ewing's sarcoma.
FAU - Berkova, A
AU  - Berkova A
AD  - Institute of Clinical Biochemistry and Laboratory Diagnostics, Charles University 
      and General Faculty Hospital, Prague, Czech Republic.
FAU - Dundr, P
AU  - Dundr P
FAU - Povysil, C
AU  - Povysil C
FAU - Melcakova, S
AU  - Melcakova S
FAU - Tvrdik, D
AU  - Tvrdik D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Czech Republic
TA  - Cesk Patol
JT  - Ceskoslovenska patologie
JID - 0050734
RN  - 0 (FLI1 protein, human)
RN  - 0 (Genetic Markers)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Retroviridae Proteins, Oncogenic)
RN  - 0 (oncogene proteins v-ets)
SB  - IM
MH  - Chromosomes, Human, Pair 22
MH  - *Genetic Markers
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Oncogene Proteins, Fusion/analysis
MH  - Paraffin Embedding
MH  - Proto-Oncogene Protein c-fli-1/genetics
MH  - RNA-Binding Protein EWS/genetics
MH  - Retroviridae Proteins, Oncogenic/genetics
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Sarcoma, Ewing/*diagnosis/genetics
MH  - Soft Tissue Neoplasms/*diagnosis/genetics
MH  - Translocation, Genetic
EDAT- 2008/09/12 09:00
MHDA- 2008/10/03 09:00
CRDT- 2008/09/12 09:00
PHST- 2008/09/12 09:00 [pubmed]
PHST- 2008/10/03 09:00 [medline]
PHST- 2008/09/12 09:00 [entrez]
PST - ppublish
SO  - Cesk Patol. 2008 Jul;44(3):67-70.