PMID- 18783794
OWN - NLM
STAT- MEDLINE
DCOM- 20090210
LR  - 20090119
IS  - 1097-6833 (Electronic)
IS  - 0022-3476 (Linking)
VI  - 154
IP  - 2
DP  - 2009 Feb
TI  - Prospective study of etiologic agents of acute gastroenteritis outbreaks in child 
      care centers.
PG  - 253-7
LID - 10.1016/j.jpeds.2008.07.057 [doi]
AB  - OBJECTIVE: To investigate the etiology of outbreaks of acute gastroenteritis 
      (AGE) in children attending childcare centers (CCCs) in North Carolina between 
      October 2005 and March 2007. STUDY DESIGN: In this prospective study of outbreaks 
      of AGE in CCCs, stool specimens from symptomatic children and environmental 
      surface swabs were tested for adenovirus group F, astrovirus, norovirus (NoV), 
      rotavirus group A, and sapovirus using real-time and conventional reverse 
      transcription-polymerase chain reaction assays, and viruses were genotyped by 
      sequencing. RESULTS: A total of 29 AGE outbreaks were evaluated, of which 13 
      (45%) were caused by a single virus, including rotavirus group A (17%), NoV 
      (10%), astrovirus (10%), and sapovirus (7%). Multiple viruses were detected in 3 
      outbreaks (10%). Environmental swabs from 13 of 22 outbreaks (59%) tested 
      positive for at least 1 virus and confirmed the findings of the same virus in the 
      fecal specimens for 10 of the outbreaks (45%). CONCLUSIONS: This study confirms 
      that viruses are major causes of AGE outbreak in CCCs. Our finding of widespread 
      environmental contamination during such outbreaks suggests the need to study the 
      effectiveness of current surface disinfectants against multiple gastroenteritis 
      viruses in CCCs.
FAU - Lyman, Whitney H
AU  - Lyman WH
AD  - Department of Environmental Sciences and Engineering, University of North 
      Carolina, Chapel Hill, NC, USA.
FAU - Walsh, Joan F
AU  - Walsh JF
FAU - Kotch, Jonathan B
AU  - Kotch JB
FAU - Weber, David J
AU  - Weber DJ
FAU - Gunn, Elizabeth
AU  - Gunn E
FAU - Vinje, Jan
AU  - Vinje J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080910
PL  - United States
TA  - J Pediatr
JT  - The Journal of pediatrics
JID - 0375410
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Acute Disease
MH  - *Child Day Care Centers
MH  - Child, Preschool
MH  - *Disease Outbreaks
MH  - Environment
MH  - Feces/virology
MH  - Gastroenteritis/*epidemiology/*virology
MH  - Humans
MH  - Infant
MH  - North Carolina/epidemiology
MH  - Prospective Studies
MH  - RNA Virus Infections/*epidemiology
MH  - RNA, Viral/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2008/09/12 09:00
MHDA- 2009/02/12 09:00
CRDT- 2008/09/12 09:00
PHST- 2008/01/30 00:00 [received]
PHST- 2008/06/11 00:00 [revised]
PHST- 2008/07/30 00:00 [accepted]
PHST- 2008/09/12 09:00 [pubmed]
PHST- 2009/02/12 09:00 [medline]
PHST- 2008/09/12 09:00 [entrez]
AID - S0022-3476(08)00674-4 [pii]
AID - 10.1016/j.jpeds.2008.07.057 [doi]
PST - ppublish
SO  - J Pediatr. 2009 Feb;154(2):253-7. doi: 10.1016/j.jpeds.2008.07.057. Epub 2008 Sep 
      10.