PMID- 18784326
OWN - NLM
STAT- MEDLINE
DCOM- 20081218
LR  - 20081031
IS  - 0890-3344 (Print)
IS  - 0890-3344 (Linking)
VI  - 24
IP  - 4
DP  - 2008 Nov
TI  - Using interphase fluorescence in situ hybridization (I-FISH) to detect the 
      transfer of infant cells during breastfeeding.
PG  - 401-5
LID - 10.1177/0890334408322264 [doi]
AB  - Using fluorochrome-labeled probes for the X and Y chromosomes, fluorescence in 
      situ hybridization (FISH) was used to test for the presence of male cells in the 
      milk of women breastfeeding male babies. After breastfeeding, the women wiped one 
      breast and collected a test sample. Wiping efficiency was evaluated in 2 mothers 
      by running a solution over the nipple after wiping. Cells were collected from 
      both the milk and the rinse samples by centrifugation. The cells were fixed to 
      glass slides for hybridization with the X and Y probes. Two cell populations were 
      observed: small female (XX) cells from the mother, and large epithelial cells 
      that were shown to be male (XY), presumably from the baby's mouth. Results varied 
      from 0 to 175 male cells detected per milliliter of breast milk. This finding 
      suggests that small molecules, including possible pathogens, in a baby's mouth, 
      might also be passed directly into the breast.
FAU - Densmore, Lezlie
AU  - Densmore L
AD  - Baystate Medical Center, 759 Chestnut Street, Springfield, MA 01199, USA.
FAU - Pflueger, Solveig M V
AU  - Pflueger SM
LA  - eng
PT  - Journal Article
DEP - 20080910
PL  - United States
TA  - J Hum Lact
JT  - Journal of human lactation : official journal of International Lactation 
      Consultant Association
JID - 8709498
MH  - Adult
MH  - *Breast Feeding
MH  - Chromosomes, Human, X/*genetics
MH  - Chromosomes, Human, Y/*genetics
MH  - Epithelial Cells
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Interphase
MH  - Male
MH  - Milk, Human/chemistry/*cytology
EDAT- 2008/09/12 09:00
MHDA- 2008/12/19 09:00
CRDT- 2008/09/12 09:00
PHST- 2008/09/12 09:00 [pubmed]
PHST- 2008/12/19 09:00 [medline]
PHST- 2008/09/12 09:00 [entrez]
AID - 0890334408322264 [pii]
AID - 10.1177/0890334408322264 [doi]
PST - ppublish
SO  - J Hum Lact. 2008 Nov;24(4):401-5. doi: 10.1177/0890334408322264. Epub 2008 Sep 
      10.