PMID- 18784756
OWN - NLM
STAT- MEDLINE
DCOM- 20081103
LR  - 20131121
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Linking)
VI  - 2
IP  - 10
DP  - 2008 Oct
TI  - Anaerobic glyoxylate cycle activity during simultaneous utilization of glycogen 
      and acetate in uncultured Accumulibacter enriched in enhanced biological 
      phosphorus removal communities.
PG  - 1040-51
LID - 10.1038/ismej.2008.45 [doi]
AB  - Enhanced biological phosphorus removal (EBPR) communities protect waterways from 
      nutrient pollution and enrich microorganisms capable of assimilating acetate as 
      polyhydroxyalkanoate (PHA) under anaerobic conditions. Accumulibacter, an 
      important uncultured polyphosphate-accumulating organism (PAO) enriched in EBPR, 
      was investigated to determine the central metabolic pathways responsible for 
      producing PHA. Acetate uptake and assimilation to PHA in Accumulibacter was 
      confirmed using fluorescence in situ hybridization (FISH)-microautoradiography 
      and post-FISH chemical staining. Assays performed with enrichments of 
      Accumulibacter using an inhibitor of glyceraldehyde-3-phosphate dehydrogenase 
      inferred anaerobic glycolysis activity. Significant decrease in anaerobic acetate 
      uptake and PHA production rates were observed using inhibitors targeting enzymes 
      within the glyoxylate cycle. Bioinformatic analysis confirmed the presence of 
      genes unique to the glyoxylate cycle (isocitrate lyase and malate synthase) and 
      gene expression analysis of isocitrate lyase demonstrated that the glyoxylate 
      cycle is likely involved in PHA production. Reduced anaerobic acetate uptake and 
      PHA production was observed after inhibition of succinate dehydrogenase and 
      upregulation of a succinate dehydrogenase gene suggested anaerobic activity. 
      Cytochrome b/b(6) activity inferred that succinate dehydrogenase activity in the 
      absence of external electron acceptors may be facilitated by a novel cytochrome 
      b/b(6) fusion protein complex that pushes electrons uphill to more 
      electronegative electron carriers. Identification of phosphoenolpyruvate 
      carboxylase and phosphoenolpyruvate carboxykinase genes in Accumulibacter 
      demonstrated the potential for interconversion of C(3) intermediates of 
      glycolysis and C(4) intermediates of the glyoxylate cycle. Our findings along 
      with previous hypotheses from analysis of microbiome data and metabolic models 
      for PAOs were used to develop a model for anaerobic carbon metabolism in 
      Accumulibacter.
FAU - Burow, Luke C
AU  - Burow LC
AD  - Advanced Water Management Centre, The University of Queensland, St Lucia, 
      Queensland, Australia.
FAU - Mabbett, Amanda N
AU  - Mabbett AN
FAU - Blackall, Linda L
AU  - Blackall LL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080911
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (Acetates)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Glyoxylates)
RN  - 27YLU75U4W (Phosphorus)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.3.3.9 (Malate Synthase)
RN  - EC 4.1.3.1 (Isocitrate Lyase)
RN  - JQ39C92HH6 (glyoxylic acid)
SB  - IM
MH  - Acetates/*metabolism
MH  - Anaerobiosis
MH  - Bacterial Proteins/genetics/metabolism
MH  - Betaproteobacteria/enzymology/genetics/isolation & purification/*metabolism
MH  - Biodegradation, Environmental
MH  - Biomass
MH  - Bioreactors/microbiology
MH  - Glycogen/*metabolism
MH  - Glyoxylates/*metabolism
MH  - Isocitrate Lyase/genetics/metabolism
MH  - Malate Synthase/genetics/metabolism
MH  - Phosphorus/*metabolism
MH  - *Water Microbiology
MH  - Water Purification
EDAT- 2008/09/12 09:00
MHDA- 2008/11/04 09:00
CRDT- 2008/09/12 09:00
PHST- 2008/09/12 09:00 [pubmed]
PHST- 2008/11/04 09:00 [medline]
PHST- 2008/09/12 09:00 [entrez]
AID - ismej200845 [pii]
AID - 10.1038/ismej.2008.45 [doi]
PST - ppublish
SO  - ISME J. 2008 Oct;2(10):1040-51. doi: 10.1038/ismej.2008.45. Epub 2008 Sep 11.