PMID- 18784832
OWN - NLM
STAT- MEDLINE
DCOM- 20081125
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 3
IP  - 9
DP  - 2008 Sep 11
TI  - Astrocyte-specific genes are generally demethylated in neural precursor cells 
      prior to astrocytic differentiation.
PG  - e3189
LID - 10.1371/journal.pone.0003189 [doi]
LID - e3189
AB  - Epigenetic changes are thought to lead to alterations in the property of cells, 
      such as differentiation potential. Neural precursor cells (NPCs) differentiate 
      only into neurons in the midgestational brain, yet they become able to generate 
      astrocytes in the late stage of development. This differentiation-potential 
      switch could be explained by epigenetic changes, since the promoters of 
      astrocyte-specific marker genes, glial fibrillary acidic protein (Gfap) and 
      S100beta, have been shown to become demethylated in late-stage NPCs prior to the 
      onset of astrocyte differentiation; however, whether demethylation occurs 
      generally in other astrocyctic genes remains unknown. Here we analyzed DNA 
      methylation changes in mouse NPCs between the mid-(E11.5) and late (E14.5) stage 
      of development by a genome-wide DNA methylation profiling method using 
      microarrays and found that many astrocytic genes are demethylated in late-stage 
      NPCs, enabling the cell to become competent to express these genes. Although 
      these genes are already demethylated in late-stage NPCs, they are not expressed 
      until cells differentiate into astrocytes. Thus, late-stage NPCs have epigenetic 
      potential which can be realized in their expression after astrocyte 
      differentiation.
FAU - Hatada, Izuho
AU  - Hatada I
AD  - Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for 
      Molecular and Cellular Regulation, Gunma University, Maebashi, Japan. 
      ihatada@showa.gunma-u.ac.jp
FAU - Namihira, Masakazu
AU  - Namihira M
FAU - Morita, Sumiyo
AU  - Morita S
FAU - Kimura, Mika
AU  - Kimura M
FAU - Horii, Takuro
AU  - Horii T
FAU - Nakashima, Kinichi
AU  - Nakashima K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080911
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (S100 Calcium Binding Protein beta Subunit)
RN  - 0 (S100 Proteins)
SB  - IM
MH  - Animals
MH  - Astrocytes/cytology/*metabolism
MH  - Binding Sites
MH  - Cell Differentiation
MH  - DNA Methylation
MH  - Epigenesis, Genetic
MH  - *Gene Expression Profiling
MH  - Genome
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Nerve Growth Factors/metabolism
MH  - Neurons/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - S100 Calcium Binding Protein beta Subunit
MH  - S100 Proteins/metabolism
MH  - Time Factors
PMC - PMC2527128
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2008/09/12 09:00
MHDA- 2008/12/17 09:00
PMCR- 2008/09/11
CRDT- 2008/09/12 09:00
PHST- 2008/04/21 00:00 [received]
PHST- 2008/08/19 00:00 [accepted]
PHST- 2008/09/12 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/09/12 09:00 [entrez]
PHST- 2008/09/11 00:00 [pmc-release]
AID - 08-PONE-RA-04396R1 [pii]
AID - 10.1371/journal.pone.0003189 [doi]
PST - epublish
SO  - PLoS One. 2008 Sep 11;3(9):e3189. doi: 10.1371/journal.pone.0003189.