PMID- 18785408
OWN - NLM
STAT- MEDLINE
DCOM- 20090707
LR  - 20080912
IS  - 1009-2587 (Print)
IS  - 1009-2587 (Linking)
VI  - 24
IP  - 2
DP  - 2008 Apr
TI  - [The influence of microtubule intervention drugs on glycolytic key enzymes in 
      myocardial cells after hypoxia].
PG  - 102-6
AB  - OBJECTIVE: To investigate the influence of microtubule intervention drugs on 
      glycolytic key enzymes in myocardial cells after hypoxia. METHODS: The primary 
      passage of cultured myocardial cells from neonatal rats were divided into A group 
      (with hypoxia), B group (with hypoxia and administration of l0 micromol/L 
      colchicine), C group (with hypoxia and administration of 5 micromol/L taxol), D 
      group (with hypoxia and administration of 10 micromol/L taxol), E group (with 
      hypoxia and administration of 15 micromol/L taxol). The morphology of microtubule 
      was observed with laser scanning microscope (LSM). The cell vitality was assayed 
      by cell counting kit (CCK). The activities of hexokinase (HK), pyruvate kinase 
      (PK), phosphofructokinase (PFK) and lactate dehydrogenase (LDH) were assayed with 
      colorimetry. RESULTS: In group B and E, the microtubule structure was damaged 
      heavily, and the cell vitality was decreased significantly [The cell vitality was 
      (89.99 +/- 3.47)% in B group and (84.56 +/- 6.61)% in E group, respectively, at 
      1.0 post hypoxia hour (PHH), and hoth values were obviously lower than that in A 
      group (97.44 +/- 1.76)%, P < 0.01]. The HK, PK and PFK activities decreased 
      obviously. The activities of HK, PK and PFK in group C were similar to those of 
      the A group. Compared with that in other groups, the degree of damage of 
      microtubule structure in D group was milden. The activities of HK, PK and PFK in 
      D group during 0.5 - 6.0 PHH were significantly higher than those in A group. The 
      activity of LDH in each group was increased after hypoxia. CONCLUSION: Proper 
      concentration of microtubule-stabilizing drugs can alleviate the damages to 
      microtubule structure, and enhance the activity of glycolytic key enzymes of 
      myocardial cells at early stage of hypoxia.
FAU - Teng, Miao
AU  - Teng M
AD  - Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, 
      Burns and Combined Injury, Third Military Medical University, Chongqing 400038, 
      PR China.
FAU - Huang, Yue-Sheng
AU  - Huang YS
FAU - Dang, Yong-Ming
AU  - Dang YM
FAU - Fang, Ya-Dong
AU  - Fang YD
FAU - Zhang, Qiong
AU  - Zhang Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Shao Shang Za Zhi
JT  - Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
JID - 100959418
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - Animals
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Glycolysis/*drug effects
MH  - Hexokinase/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Microtubules/*drug effects/metabolism
MH  - Myocytes, Cardiac/enzymology/*metabolism
MH  - Phosphofructokinase-1/metabolism
MH  - Pyruvate Kinase/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2008/09/13 09:00
MHDA- 2009/07/08 09:00
CRDT- 2008/09/13 09:00
PHST- 2008/09/13 09:00 [pubmed]
PHST- 2009/07/08 09:00 [medline]
PHST- 2008/09/13 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Shao Shang Za Zhi. 2008 Apr;24(2):102-6.