PMID- 18786435
OWN - NLM
STAT- MEDLINE
DCOM- 20080926
LR  - 20191210
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 186
IP  - 1
DP  - 2008 Oct
TI  - Investigation of bone marrow involvement in malignant lymphoma using fluorescence 
      in situ hybridization: possible utility in the detection of micrometastasis.
PG  - 1-5
LID - 10.1016/j.cancergencyto.2008.04.012 [doi]
AB  - We evaluated the usefulness of interphase fluorescence in situ hybridization 
      (FISH) for the detection of bone marrow involvement of lymphoma, comparing the 
      results with those of microscopic examination. Bone marrow aspirates obtained for 
      staging work-up from 150 patients with non-Hodgkin lymphoma were used in this 
      study. Interphase FISH study using four probes and conventional G-banding were 
      performed on bone marrow aspirates. The four probes included locus specific 
      identifier (LSI) immunoglobulin heavy chain (IGH) dual-color break-apart 
      rearrangement probe, an LSI p16 SpectrumOrange/CEP 9 SpectrumGreen probe, an LSI 
      BCL6 dual-color break-apart rearrangement probe. Among 150 cases, 29 cases 
      (19.3%) showed infiltration of neoplastic lymphoid cells by microscopic 
      examination. Chromosomal aberrations were detected by FISH in eight patients and 
      by conventional cytogenetic study in three patients. FISH study showed 14q32 
      rearrangement in four patients (4/126, 3.2%), 9q21 rearrangement in no patients 
      (0/144, 0%), 3q27 rearrangement in four patients (4/131. 3.1%), and a gain of 
      1q21q32 in two patients (2/115, 1.7%). Among eight patients with abnormal FISH 
      patterns, six had normal karyotypes or no analyzable metaphase according to the 
      conventional cytogenetic study. Seven patients with FISH abnormality showed bone 
      marrow involvement of lymphoma by microscopic examination. One patient, who was 
      defined as having no evidence of bone marrow involvement by microscopic 
      examination, showed a 3q27 aberration in the FISH study. Although the number of 
      patients with BM involvement that was detected by FISH was low, abnormal FISH 
      patterns were detected in six patients who did not have abnormal karyotypes. 
      Therefore, FISH analysis would be beneficial in cytogenetic diagnosis and 
      follow-up study of minimal residual diseases, once the cytogenetic changes are 
      detected at initial diagnosis.
FAU - Huh, Hee Jin
AU  - Huh HJ
AD  - Department of Laboratory Medicine, Dongguk University, College of Medicine, 
      Siksa-dong, Ilsandong-gu, Goyang, Gyeonggi-do, Republic of Korea.
FAU - Min, Hyun Chung
AU  - Min HC
FAU - Cho, Han Ik
AU  - Cho HI
FAU - Chae, Seok Lae
AU  - Chae SL
FAU - Lee, Dong Soon
AU  - Lee DS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow/*pathology
MH  - Bone Marrow Examination/*methods
MH  - Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 1/genetics/ultrastructure
MH  - Chromosomes, Human, Pair 14/genetics/ultrastructure
MH  - Chromosomes, Human, Pair 3/genetics/ultrastructure
MH  - Chromosomes, Human, Pair 9/genetics/ultrastructure
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Karyotyping
MH  - Lymphoma, Non-Hodgkin/blood/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging/*methods
EDAT- 2008/09/13 09:00
MHDA- 2008/09/27 09:00
CRDT- 2008/09/13 09:00
PHST- 2008/01/31 00:00 [received]
PHST- 2008/04/03 00:00 [revised]
PHST- 2008/04/14 00:00 [accepted]
PHST- 2008/09/13 09:00 [pubmed]
PHST- 2008/09/27 09:00 [medline]
PHST- 2008/09/13 09:00 [entrez]
AID - S0165-4608(08)00266-5 [pii]
AID - 10.1016/j.cancergencyto.2008.04.012 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2008 Oct;186(1):1-5. doi: 
      10.1016/j.cancergencyto.2008.04.012.