PMID- 18787609
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100122
LR  - 20211020
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 1
IP  - 4
DP  - 2008 Jan 1
TI  - Amplification of EMSY gene in a subset of sporadic pancreatic adenocarcinomas.
PG  - 343-51
AB  - Mutations in the breast cancer susceptibility gene 2 (BRCA2) are commonly found 
      in familial pancreatic cancer. Recently, EMSY (11q13.5) has been described as a 
      BRCA2 interacting protein capable of binding and inactivating the protein domain 
      encoded by exon 3 of the BRCA2 gene. Amplification of EMSY occurs in 13% of 
      sporadic breast cancers and is directly linked to increased expression. Here we 
      investigate the amplification status of this new potential oncogene in 59 
      sporadic pancreatic cancers using fluorescence in situ hybridization (FISH) and 
      tissue microarray (TMA). Real-time quantitative RT-PCR was performed on 20 
      pancreatic cancer cell lines and overexpression was calculated using the 
      delta-delta-Ct-method. Amplification of EMSY was found in 8/59 cases (13.6%). 
      9/20 (45%) cell line samples showed overexpression of EMSY. In conclusion, 
      sporadic pancreatic cancer shows amplification of EMSY at prevalence similar to 
      that found in other cancers.
FAU - van Hattem, W Arnout
AU  - van Hattem WA
AD  - Departments of Oncology Center, The Sol Goldman Pancreatic Research Center, The 
      Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; Department 
      of Pathology, Academic Medical Center, Amsterdam, the Netherlands, and the 
      University Medical Center, Utrecht, the Netherlands.
FAU - Carvalho, Ralph
AU  - Carvalho R
FAU - Li, Ang
AU  - Li A
FAU - Offerhaus, G Johan A
AU  - Offerhaus GJ
FAU - Goggins, Michael
AU  - Goggins M
LA  - eng
GR  - P50 CA062924/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20080101
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC2480540
OTO - NOTNLM
OT  - BRCA2
OT  - EMSY
OT  - FISH
OT  - Pancreatic cancer
OT  - amplification
EDAT- 2008/09/13 09:00
MHDA- 2008/09/13 09:01
PMCR- 2008/01/01
CRDT- 2008/09/13 09:00
PHST- 2007/08/10 00:00 [received]
PHST- 2007/08/28 00:00 [accepted]
PHST- 2008/09/13 09:00 [pubmed]
PHST- 2008/09/13 09:01 [medline]
PHST- 2008/09/13 09:00 [entrez]
PHST- 2008/01/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2008 Jan 1;1(4):343-51.