PMID- 18787640
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20090429
LR  - 20211020
IS  - 0044-5991 (Print)
IS  - 1347-5800 (Electronic)
IS  - 0044-5991 (Linking)
VI  - 41
IP  - 4
DP  - 2008 Aug 28
TI  - Pentosidine accumulation in human oocytes and their correlation to age-related 
      apoptosis.
PG  - 97-104
LID - 10.1267/ahc.08014 [doi]
AB  - Age-related atresia of ovarian follicles is characterized by apoptosis of the 
      constituent cells. Recent studies have indicated that dysfunction of the 
      proteasome and endoplasmic reticulum and subsequent apoptosis in the presence of 
      oxidative stress have relevance to aging. The aim of this study was to assess the 
      involvement of these processes in age-related follicular atresia. Formalin-fixed, 
      paraffin-embedded sections of ovaries obtained at surgery from 74 women (age: 
      21-54 y) were examined with the terminal deoxynucleotidyl transferase-mediated, 
      dUTP-biotin nick-end labeling (TUNEL) method and an immunohistochemical 
      technique. Primary antibodies used in immunohistochemistry were against 
      pentosidine, ubiquitin and caspase 12. Histological localization of these 
      substances in oocytes was observed by light microscopy, and labeling indices of 
      these cells were evaluated by regression analysis. Positive signals for 
      pentosidine, ubiquitin, caspase 12, and TUNEL were detectable in oocytes of the 
      primordial, primary and their atretic follicles. Regression analysis revealed an 
      age-related increase in the labeling indices for pentosidine, ubiquitin, caspase 
      12, and TUNEL. These results suggest that pentosidine accumulation in human 
      oocytes is related to apoptosis and increases with age. Further studies will be 
      necessary to clarify the involvement of pentosidine accumulation, proteasome 
      inhibition, and endoplasmic reticulum stress in age-related apoptosis of oocytes 
      in human ovaries.
FAU - Matsumine, Miki
AU  - Matsumine M
AD  - Department of Obstetrics and Gynecology, Tokyo Women's Medical University, 8-1 
      Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
FAU - Shibata, Noriyuki
AU  - Shibata N
FAU - Ishitani, Ken
AU  - Ishitani K
FAU - Kobayashi, Makio
AU  - Kobayashi M
FAU - Ohta, Hiroaki
AU  - Ohta H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Acta Histochem Cytochem
JT  - Acta histochemica et cytochemica
JID - 0147110
PMC - PMC2532604
OTO - NOTNLM
OT  - aging
OT  - apoptosis
OT  - oocyte
OT  - oxidative stress
OT  - proteasome inhibition
EDAT- 2008/09/13 09:00
MHDA- 2008/09/13 09:01
PMCR- 2008/08/28
CRDT- 2008/09/13 09:00
PHST- 2008/04/11 00:00 [received]
PHST- 2008/06/05 00:00 [accepted]
PHST- 2008/09/13 09:00 [pubmed]
PHST- 2008/09/13 09:01 [medline]
PHST- 2008/09/13 09:00 [entrez]
PHST- 2008/08/28 00:00 [pmc-release]
AID - AHC08014 [pii]
AID - 10.1267/ahc.08014 [doi]
PST - ppublish
SO  - Acta Histochem Cytochem. 2008 Aug 28;41(4):97-104. doi: 10.1267/ahc.08014.