PMID- 18791173
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20220330
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 50
IP  - 2
DP  - 2009 Feb
TI  - Beta-amyloid deposition and functional impairment in the retina of the 
      APPswe/PS1DeltaE9 transgenic mouse model of Alzheimer's disease.
PG  - 793-800
LID - 10.1167/iovs.08-2384 [doi]
AB  - PURPOSE: To determine whether beta-amyloid (Abeta) deposition affects the 
      structure and function of the retina of the APPswe/PS1DeltaE9 transgenic (tg) 
      mouse model of Alzheimer's disease. METHODS: Retinas from 12- to 19-month old 
      APPswe/PS1DeltaE9 tg and age-matched non-transgenic (ntg) littermates were single 
      or double stained with thioflavine-S and antibodies against Abeta, glial 
      fibrillar acidic protein (GFAP), microglial marker F4/80, choline 
      acetyltransferase (ChAT), and syntaxin 1. Quantification of thioflavine-S 
      positive plaques and retinal layer thickness was analyzed semi-quantitatively, 
      whereas microglial cell size and levels of F4/80 immunoreactivity were evaluated 
      using a densitometry program. Scotopic electroretinogram (ERG) recording was used 
      to investigate retinal physiology in these mice. RESULTS: Thioflavine-S positive 
      plaques appeared at 12 months in the retinas of APPswe/PS1DeltaE9 tg mice with 
      the majority of plaques in the outer and inner plexiform layers. Plaques were 
      embedded in the inner plexiform layer strata displaying syntaxin 1 and ChAT. The 
      number and size of the plaques in the retina increased with age. Plaques appeared 
      earlier and in greater numbers in females than in male tg littermate mice. 
      Microglial activity was significantly increased in the retinas of 
      APPswe/PS1DeltaE9 tg mice. Although we did not detect neuronal degeneration in 
      the retina, ERG recordings revealed a significant reduction in the amplitudes of 
      a- and b-waves in aged APPswe/PS1DeltaE9 tg compared to ntg littermates. 
      CONCLUSIONS: The present findings suggest that Abeta deposition disrupts retinal 
      structure and may contribute to the visual deficits seen in aged 
      APPswe/PS1DeltaE9 tg mice. Whether Abeta is involved in other forms of 
      age-related retinal dysfunction is unclear.
FAU - Perez, Sylvia E
AU  - Perez SE
AD  - Department of Neurological Sciences, Rush University Medical Center, Chicago, 
      Illinois, USA.
FAU - Lumayag, Stephen
AU  - Lumayag S
FAU - Kovacs, Beatrix
AU  - Kovacs B
FAU - Mufson, Elliott J
AU  - Mufson EJ
FAU - Xu, Shunbin
AU  - Xu S
LA  - eng
GR  - P01 AG014449/AG/NIA NIH HHS/United States
GR  - R01 AG010668/AG/NIA NIH HHS/United States
GR  - R01 AG043375/AG/NIA NIH HHS/United States
GR  - AG10668/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080912
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Benzothiazoles)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Presenilin-1)
RN  - 0 (Syntaxin 1)
RN  - 0 (Thiazoles)
RN  - 0 (monocyte-macrophage differentiation antigen)
RN  - 2390-54-7 (thioflavin T)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Aging/physiology
MH  - Alzheimer Disease/genetics/*metabolism/physiopathology
MH  - Amyloid beta-Peptides/*metabolism
MH  - Amyloid beta-Protein Precursor/genetics
MH  - Animals
MH  - Antigens, Differentiation/metabolism
MH  - Benzothiazoles
MH  - Choline O-Acetyltransferase/metabolism
MH  - *Disease Models, Animal
MH  - Electroretinography
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Fluorescent Dyes/metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Gliosis/*metabolism/physiopathology
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Microscopy, Confocal
MH  - Presenilin-1/genetics
MH  - Retina/physiopathology
MH  - Retinal Diseases/*metabolism/*physiopathology
MH  - Syntaxin 1/metabolism
MH  - Thiazoles/metabolism
PMC - PMC3697019
MID - NIHMS477992
EDAT- 2008/09/16 09:00
MHDA- 2009/02/07 09:00
PMCR- 2013/07/01
CRDT- 2008/09/16 09:00
PHST- 2008/09/16 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
PHST- 2008/09/16 09:00 [entrez]
PHST- 2013/07/01 00:00 [pmc-release]
AID - iovs.08-2384 [pii]
AID - 10.1167/iovs.08-2384 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2009 Feb;50(2):793-800. doi: 10.1167/iovs.08-2384. 
      Epub 2008 Sep 12.