PMID- 18791231
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20221207
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 180
IP  - 3
DP  - 2008 Nov
TI  - Cross-species bacterial artificial chromosome-fluorescence in situ hybridization 
      painting of the tomato and potato chromosome 6 reveals undescribed chromosomal 
      rearrangements.
PG  - 1319-28
LID - 10.1534/genetics.108.093211 [doi]
AB  - Ongoing genomics projects of tomato (Solanum lycopersicum) and potato (S. 
      tuberosum) are providing unique tools for comparative mapping studies in 
      Solanaceae. At the chromosomal level, bacterial artificial chromosomes (BACs) can 
      be positioned on pachytene complements by fluorescence in situ hybridization 
      (FISH) on homeologous chromosomes of related species. Here we present results of 
      such a cross-species multicolor cytogenetic mapping of tomato BACs on potato 
      chromosomes 6 and vice versa. The experiments were performed under low 
      hybridization stringency, while blocking with Cot-100 was essential in 
      suppressing excessive hybridization of repeat signals in both within-species FISH 
      and cross-species FISH of tomato BACs. In the short arm we detected a large 
      paracentric inversion that covers the whole euchromatin part with breakpoints 
      close to the telomeric heterochromatin and at the border of the short arm 
      pericentromere. The long arm BACs revealed no deviation in the colinearity 
      between tomato and potato. Further comparison between tomato cultivars Cherry 
      VFNT and Heinz 1706 revealed colinearity of the tested tomato BACs, whereas one 
      of the six potato clones (RH98-856-18) showed minor putative rearrangements 
      within the inversion. Our results present cross-species multicolor BAC-FISH as a 
      unique tool for comparative genetic studies across Solanum species.
FAU - Tang, Xiaomin
AU  - Tang X
AD  - Wageningen-UR Plant Breeding, PB Wageningen, The Netherlands.
FAU - Szinay, Dora
AU  - Szinay D
FAU - Lang, Chunting
AU  - Lang C
FAU - Ramanna, Munikote S
AU  - Ramanna MS
FAU - van der Vossen, Edwin A G
AU  - van der Vossen EA
FAU - Datema, Erwin
AU  - Datema E
FAU - Lankhorst, Rene Klein
AU  - Lankhorst RK
FAU - de Boer, Jan
AU  - de Boer J
FAU - Peters, Sander A
AU  - Peters SA
FAU - Bachem, Christian
AU  - Bachem C
FAU - Stiekema, Willem
AU  - Stiekema W
FAU - Visser, Richard G F
AU  - Visser RG
FAU - de Jong, Hans
AU  - de Jong H
FAU - Bai, Yuling
AU  - Bai Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080914
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Plant/*genetics
MH  - DNA, Plant/genetics
MH  - Genetic Markers
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Solanum lycopersicum/*genetics
MH  - Physical Chromosome Mapping/*methods
MH  - Solanum tuberosum/*genetics
MH  - Species Specificity
PMC - PMC2581937
EDAT- 2008/09/16 09:00
MHDA- 2009/02/07 09:00
PMCR- 2009/02/01
CRDT- 2008/09/16 09:00
PHST- 2008/09/16 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
PHST- 2008/09/16 09:00 [entrez]
PHST- 2009/02/01 00:00 [pmc-release]
AID - genetics.108.093211 [pii]
AID - gen18031319 [pii]
AID - 10.1534/genetics.108.093211 [doi]
PST - ppublish
SO  - Genetics. 2008 Nov;180(3):1319-28. doi: 10.1534/genetics.108.093211. Epub 2008 
      Sep 14.