PMID- 18793720
OWN - NLM
STAT- MEDLINE
DCOM- 20090306
LR  - 20131121
IS  - 0923-2508 (Print)
IS  - 0923-2508 (Linking)
VI  - 159
IP  - 9-10
DP  - 2008 Nov-Dec
TI  - Bacterial and H2O2 stress-induced apoptosis-like events in Cryptococcus 
      neoformans.
PG  - 628-34
LID - 10.1016/j.resmic.2008.07.006 [doi]
AB  - Cell death induced by adherence of Staphylococcus aureus was observed in 
      Cryptococcus neoformans. To investigate this death mechanism, we examined three 
      factors: actin dynamics using rhodamine-phalloidin staining, accumulation of 
      reactive oxygen species (ROS) via conversion of 2',7'-dichlorofluorescein 
      diacetate to 2',7'-dichlorofluorescein, and DNA fragmentation via terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). In 
      co-culture, actin visibly clumped and the percentage of ROS- and TUNEL-positive 
      cells increased. The same cellular events were observed in C. neoformans after 
      addition of hydrogen peroxide (H2O2), creating a bacterial cell-free system. 
      After H2O2 treatment and cell disruption, C. neoformans proteins in soluble 
      fraction were separated by two-dimensional (2-D) electrophoresis. A 
      voltage-dependent ion-selective channel was identified as one of the 
      stress-enhanced proteins. Thus, the death of C. neoformans was accompanied by 
      decreased actin turnover, enhanced ROS accumulation, and DNA fragmentation. Cell 
      death appeared similar to apoptosis via mitochondrial pathways.
FAU - Ikeda, Reiko
AU  - Ikeda R
AD  - Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, 
      Kiyose, Tokyo 204-8588, Japan. ikeda@my-pharm.ac.jp
FAU - Sawamura, Kanako
AU  - Sawamura K
LA  - eng
PT  - Journal Article
DEP - 20080829
PL  - France
TA  - Res Microbiol
JT  - Research in microbiology
JID - 8907468
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Voltage-Dependent Anion Channels)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - *Apoptosis/drug effects
MH  - Bacterial Adhesion/*physiology
MH  - Cryptococcus neoformans/drug effects/growth & development/metabolism/*physiology
MH  - DNA Fragmentation
MH  - Heat-Shock Response
MH  - Hydrogen Peroxide/metabolism/*pharmacology
MH  - *Oxidative Stress
MH  - Reactive Oxygen Species/metabolism
MH  - Staphylococcus aureus/*physiology
MH  - Voltage-Dependent Anion Channels
EDAT- 2008/09/17 09:00
MHDA- 2009/03/07 09:00
CRDT- 2008/09/17 09:00
PHST- 2008/02/21 00:00 [received]
PHST- 2008/07/22 00:00 [revised]
PHST- 2008/07/26 00:00 [accepted]
PHST- 2008/09/17 09:00 [pubmed]
PHST- 2009/03/07 09:00 [medline]
PHST- 2008/09/17 09:00 [entrez]
AID - S0923-2508(08)00134-4 [pii]
AID - 10.1016/j.resmic.2008.07.006 [doi]
PST - ppublish
SO  - Res Microbiol. 2008 Nov-Dec;159(9-10):628-34. doi: 10.1016/j.resmic.2008.07.006. 
      Epub 2008 Aug 29.