PMID- 18793839 OWN - NLM STAT- MEDLINE DCOM- 20081229 LR - 20081112 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 100 IP - 3 DP - 2009 Feb TI - Assessing the impact of composting and vermicomposting on bacterial community size and structure, and microbial functional diversity of an olive-mill waste. PG - 1319-26 LID - 10.1016/j.biortech.2008.08.014 [doi] AB - The aim of this study was to couple biochemical and molecular methodologies for evaluating the impact of two recycling technologies (composting and vermicomposting) on a toxic organic waste. To do this, six enzyme activities controlling the key metabolic pathways of the breakdown of organic matter, real-time PCR assays targeting 16S rRNA genes, and denaturing gradient gel electrophoresis (DGGE) profiling-sequence analysis of PCR-amplified 16S rRNA fragments have been used to determine the functional diversity, bacterial number, and bacterial community structure, respectively, in a mixture of olive waste and sheep manure, and in the derived compost and vermicompost. Both the recycling technologies were effective in activating the microbial parameters of the toxic waste, the vermicomposting being the best process to produce greater bacterial diversity, greater bacterial numbers and greater functional diversity. Although several identical populations were detected in the processed and non-processed materials, each technology modified the original microbial communities of the waste in a diverse way, indicating the different roles of each one in the bacterial selection. FAU - Vivas, A AU - Vivas A AD - Department of Environmental Protection, Estacion Experimental del Zaidin, CSIC, Granada, Spain. FAU - Moreno, B AU - Moreno B FAU - Garcia-Rodriguez, S AU - Garcia-Rodriguez S FAU - Benitez, E AU - Benitez E LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080914 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Organic Chemicals) SB - IM MH - Bacteria, Aerobic/classification/*isolation & purification/metabolism MH - Bioreactors/*microbiology MH - Colony Count, Microbial/*methods MH - Computer Simulation MH - *Models, Biological MH - Olea/*chemistry MH - Organic Chemicals/isolation & purification/*metabolism MH - Refuse Disposal/*methods MH - *Soil Microbiology EDAT- 2008/09/17 09:00 MHDA- 2008/12/30 09:00 CRDT- 2008/09/17 09:00 PHST- 2008/06/20 00:00 [received] PHST- 2008/08/05 00:00 [revised] PHST- 2008/08/07 00:00 [accepted] PHST- 2008/09/17 09:00 [pubmed] PHST- 2008/12/30 09:00 [medline] PHST- 2008/09/17 09:00 [entrez] AID - S0960-8524(08)00710-4 [pii] AID - 10.1016/j.biortech.2008.08.014 [doi] PST - ppublish SO - Bioresour Technol. 2009 Feb;100(3):1319-26. doi: 10.1016/j.biortech.2008.08.014. Epub 2008 Sep 14.