PMID- 18794152
OWN - NLM
STAT- MEDLINE
DCOM- 20081014
LR  - 20211020
IS  - 1538-7445 (Electronic)
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 68
IP  - 18
DP  - 2008 Sep 15
TI  - A fluorescence in situ hybridization screen for E26 transformation-specific 
      aberrations: identification of DDX5-ETV4 fusion protein in prostate cancer.
PG  - 7629-37
LID - 10.1158/0008-5472.CAN-08-2014 [doi]
AB  - Recurrent gene fusions involving E26 transformation-specific (ETS) transcription 
      factors ERG, ETV1, ETV4, or ETV5 have been identified in 40% to 70% of prostate 
      cancers. Here, we used a comprehensive fluorescence in situ hybridization (FISH) 
      split probe strategy interrogating all 27 ETS family members and their five known 
      5' fusion partners in a cohort of 110 clinically localized prostate cancer 
      patients. Gene rearrangements were only identified in ETS genes that were 
      previously implicated in prostate cancer gene fusions including ERG, ETV1, and 
      ETV4 (43%, 5%, and 5%, respectively), suggesting that a substantial fraction of 
      prostate cancers (estimated at 30-60%) cannot be attributed to an ETS gene 
      fusion. Among the known 5' gene fusion partners, TMPRSS2 was rearranged in 47% of 
      cases followed by SLC45A3, HNRPA2B1, and C15ORF21 in 2%, 1%, and 1% of cases, 
      respectively. Based on this comprehensive FISH screen, we have made four 
      noteworthy observations. First, by screening the entire ETS transcription factor 
      family for rearrangements, we found that a large fraction of prostate cancers 
      (44%) cannot be ascribed to an ETS gene fusion, an observation which will 
      stimulate research into identifying recurrent non-ETS aberrations in prostate 
      cancers. Second, we identified SLC45A3 as a novel 5' fusion partner of ERG; 
      previously, TMPRSS2 was the only described 5' partner of ERG. Third, we 
      identified two prostate-specific, androgen-induced genes, FLJ35294 and CANT1, as 
      5' partners to ETV1 and ETV4. Fourth, we identified a ubiquitously expressed, 
      androgen-insensitive gene, DDX5, fused in frame with ETV4, leading to the 
      expression of a DDX5-ETV4 fusion protein.
FAU - Han, Bo
AU  - Han B
AD  - Michigan Center for Translational Pathology, University of Michigan Medical 
      School, Ann Arbor, Michigan 48109, USA.
FAU - Mehra, Rohit
AU  - Mehra R
FAU - Dhanasekaran, Saravana M
AU  - Dhanasekaran SM
FAU - Yu, Jindan
AU  - Yu J
FAU - Menon, Anjana
AU  - Menon A
FAU - Lonigro, Robert J
AU  - Lonigro RJ
FAU - Wang, Xiaosong
AU  - Wang X
FAU - Gong, Yusong
AU  - Gong Y
FAU - Wang, Lei
AU  - Wang L
FAU - Shankar, Sunita
AU  - Shankar S
FAU - Laxman, Bharathi
AU  - Laxman B
FAU - Shah, Rajal B
AU  - Shah RB
FAU - Varambally, Sooryanarayana
AU  - Varambally S
FAU - Palanisamy, Nallasivam
AU  - Palanisamy N
FAU - Tomlins, Scott A
AU  - Tomlins SA
FAU - Kumar-Sinha, Chandan
AU  - Kumar-Sinha C
FAU - Chinnaiyan, Arul M
AU  - Chinnaiyan AM
LA  - eng
SI  - GENBANK/EU693077
SI  - GENBANK/EU693078
SI  - GENBANK/EU693079
SI  - GENBANK/FLJ35294
GR  - P50 CA069568-110020/CA/NCI NIH HHS/United States
GR  - R01 CA102872/CA/NCI NIH HHS/United States
GR  - P50CA69568/CA/NCI NIH HHS/United States
GR  - R01 CA097063-01/CA/NCI NIH HHS/United States
GR  - P50 CA069568/CA/NCI NIH HHS/United States
GR  - U01 CA111275/CA/NCI NIH HHS/United States
GR  - R01 CA097063/CA/NCI NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - U01 CA111275-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Adenovirus E1A Proteins)
RN  - 0 (ETV4 protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (Transcription Factors)
RN  - EC 3.6.1.- (Ddx5 protein, human)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Adenovirus E1A Proteins/*genetics
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Cell Transformation, Neoplastic/*genetics
MH  - DEAD-box RNA Helicases/*genetics
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Prostatic Neoplasms/*genetics
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins c-ets
MH  - Transcription Factors/genetics
PMC - PMC2760292
MID - NIHMS134703
EDAT- 2008/09/17 09:00
MHDA- 2008/10/15 09:00
PMCR- 2009/10/12
CRDT- 2008/09/17 09:00
PHST- 2008/09/17 09:00 [pubmed]
PHST- 2008/10/15 09:00 [medline]
PHST- 2008/09/17 09:00 [entrez]
PHST- 2009/10/12 00:00 [pmc-release]
AID - 68/18/7629 [pii]
AID - 10.1158/0008-5472.CAN-08-2014 [doi]
PST - ppublish
SO  - Cancer Res. 2008 Sep 15;68(18):7629-37. doi: 10.1158/0008-5472.CAN-08-2014.