PMID- 18795626
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20080917
IS  - 1000-503X (Print)
IS  - 1000-503X (Linking)
VI  - 30
IP  - 4
DP  - 2008 Aug
TI  - [Detection of deletion of the long arm of chromosome 13 and translocation of 
      immunoglobulin heavy chain gene by interphase fluorescence in situ hybridization 
      in patients with multiple myeloma].
PG  - 485-90
AB  - OBJECTIVE: To investigate the clinical significance of the deletion of the long 
      arm of chromosome 13 [del (13 q) ] and translocation of immunoglobulin heavy 
      chain gene [t (14 q) I in multiple myeloma (MM) patients. METHODS: Myeloma cells 
      were isolated from hone marrow by direct immunomagnetic cell sorting and 
      interphase fluorescence in situ hybridization (FISH) was performed in 24 MM 
      patients to detect del (l3q) and t (l4q). RESULTS: The positive rates of del 
      (l3q) and t (l4q) were 45.83% and 37.50% respectively. Five patients (20.83%) had 
      both two abnormalities and 15 patients (62.50%) had at least one abnormality. 
      Univariate analysis showed that the positive rates of del (l3q) were 35.71% and 
      66.67% in responders and non-responders (P = 0.214) and the positive rates of t 
      (l4q) were 21.43% and 66. 67% in responders and non-responders (P = 0.077). 
      Multivariate analysis showed that del (13q) (OR = 5.761, 95% CI 0.500-66.391, P = 
      0.160), t (14q) (OR = 6.576, 95% CI 0.580-74.614, P = 0.129), and corrected serum 
      calcium level (OR = 8.080, 95% CI 0.738-88.427, P = 0.087) were relatively 
      independent negative factors for response to therapy, with the corrected serum 
      calcium level being the strongest reversely-correlated factor. CONCLUSIONS: 
      Interphase FISH is a sensitive method to investigate the cytogenetics of MM. Del 
      (13q), t (14q), and corrected serum calcium level can be used to predict 
      treatment response and prognosis.
FAU - Sun, Wan-ling
AU  - Sun WL
AD  - Department of Hematology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
FAU - Wu, Yong-ji
AU  - Wu YJ
FAU - Li, Hui
AU  - Li H
FAU - Wang, Xuan
AU  - Wang X
FAU - Zhuang, Jun-ling
AU  - Zhuang JL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao
JT  - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
JID - 8006230
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Female
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - *Translocation, Genetic
EDAT- 2008/09/18 09:00
MHDA- 2010/06/04 06:00
CRDT- 2008/09/18 09:00
PHST- 2008/09/18 09:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
PHST- 2008/09/18 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008 Aug;30(4):485-90.