PMID- 18796637
OWN - NLM
STAT- MEDLINE
DCOM- 20090415
LR  - 20211020
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 20
IP  - 9
DP  - 2008 Sep
TI  - LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-box protein involved in 
      light-dependent development and gene expression, undergoes COP1-mediated 
      ubiquitination.
PG  - 2324-38
LID - 10.1105/tpc.108.061747 [doi]
AB  - B-box containing proteins play an important role in light signaling in plants. 
      Here, we identify LIGHT-REGULATED ZINC FINGER1/SALT TOLERANCE HOMOLOG3 (STH3), a 
      B-box encoding gene that genetically interacts with two key regulators of light 
      signaling, ELONGATED HYPOCOTYL5 (HY5) and CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). 
      STH3 physically interacts with HY5 in vivo and shows a COP1-dependent 
      localization to nuclear speckles when coexpressed with COP1 in plant cells. A 
      T-DNA insertion mutant, sth3, is hyposensitive to high fluence blue, red, and 
      far-red light and has elongated hypocotyls under short days. Analyses of double 
      mutants between sth3, sth2, and hy5 suggest that they have partially overlapping 
      functions. Interestingly, functional assays in protoplasts suggest that STH3 can 
      activate transcription both independently and together with STH2 through the 
      G-box promoter element. Furthermore, sth3 suppresses the cop1 hypocotyl phenotype 
      in the dark as well as the anthocyanin accumulation in the light. Finally, COP1 
      ubiquitinates STH3 in vitro, suggesting that STH3 is regulated by COP1. In 
      conclusion, we have identified STH3 as a positive regulator of photomorphogenesis 
      acting in concert with STH2 and HY5, while also being a target of COP1-mediated 
      ubiquitination.
FAU - Datta, Sourav
AU  - Datta S
AD  - Department of Cell and Molecular Biology, Gothenburg University, 405 30 
      Gothenburg, Sweden.
FAU - Johansson, Henrik
AU  - Johansson H
FAU - Hettiarachchi, Chamari
AU  - Hettiarachchi C
FAU - Irigoyen, Maria Luisa
AU  - Irigoyen ML
FAU - Desai, Mintu
AU  - Desai M
FAU - Rubio, Vicente
AU  - Rubio V
FAU - Holm, Magnus
AU  - Holm M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080916
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (HY5 protein, Arabidopsis)
RN  - 0 (LZF1 protein, Arabidopsis)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/*genetics/growth & development/metabolism
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Basic-Leucine Zipper Transcription Factors/genetics/metabolism
MH  - Gene Expression Regulation, Developmental/*radiation effects
MH  - Gene Expression Regulation, Plant/*radiation effects
MH  - *Light
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/metabolism
MH  - Protein Binding/radiation effects
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/genetics/metabolism
MH  - Two-Hybrid System Techniques
MH  - Ubiquitin-Protein Ligases
MH  - Ubiquitination/radiation effects
PMC - PMC2570732
EDAT- 2008/09/18 09:00
MHDA- 2009/04/16 09:00
PMCR- 2009/09/01
CRDT- 2008/09/18 09:00
PHST- 2008/09/18 09:00 [pubmed]
PHST- 2009/04/16 09:00 [medline]
PHST- 2008/09/18 09:00 [entrez]
PHST- 2009/09/01 00:00 [pmc-release]
AID - tpc.108.061747 [pii]
AID - 061747 [pii]
AID - 10.1105/tpc.108.061747 [doi]
PST - ppublish
SO  - Plant Cell. 2008 Sep;20(9):2324-38. doi: 10.1105/tpc.108.061747. Epub 2008 Sep 
      16.