PMID- 18796707 OWN - NLM STAT- MEDLINE DCOM- 20081027 LR - 20200302 IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 89 IP - Pt 10 DP - 2008 Oct TI - Dendritic cells mediate herpes simplex virus infection and transmission through the C-type lectin DC-SIGN. PG - 2398-2409 LID - 10.1099/vir.0.2008/003129-0 [doi] AB - Dendritic cells (DCs) are essential for the induction of specific immune responses against invading pathogens. Herpes simplex virus (HSV) is a common human pathogen that causes painful but mild infections of the skin and mucosa, and which results in latency and recurrent infections. Of the two HSV subtypes described, HSV-1 causes mainly oral-facial lesions, whilst HSV-2 is associated with genital herpes. DCs are involved in HSV-induced immune suppression, but little is known about the molecular interactions between DCs and HSV. This study demonstrated that HSV-1 and -2 both interact with the DC-specific C-type lectin DC-SIGN. Further analyses demonstrated that DC-SIGN interacts with the HSV glycoproteins gB and gC. Binding of HSV-1 to immature DCs depended on both DC-SIGN and heparan sulfate proteoglycans. Strikingly, HSV-1 infection of DCs was almost completely inhibited by blocking antibodies against DC-SIGN. Thus, DC-SIGN is an important attachment receptor for HSV-1 on immature DCs and enhances infection of DCs in cis. In addition, DC-SIGN captures HSV-1 for transmission to permissive target cells. These data strongly suggest that DC-SIGN is a potential target to prevent HSV infection and virus dissemination. Further studies will show whether these interactions are involved in HSV-induced immune suppression. FAU - de Jong, Marein A W P AU - de Jong MAWP AD - Department of Molecular Cell Biology and Immunology, VU University Medical Center Amsterdam, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands. FAU - de Witte, Lot AU - de Witte L AD - Department of Molecular Cell Biology and Immunology, VU University Medical Center Amsterdam, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands. FAU - Bolmstedt, Anders AU - Bolmstedt A AD - Department of Clinical Virology, University of Goteborg, Guldhedsgatan 10B, S-413 46 Goteborg, Sweden. FAU - van Kooyk, Yvette AU - van Kooyk Y AD - Department of Molecular Cell Biology and Immunology, VU University Medical Center Amsterdam, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands. FAU - Geijtenbeek, Teunis B H AU - Geijtenbeek TBH AD - Department of Molecular Cell Biology and Immunology, VU University Medical Center Amsterdam, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (Cell Adhesion Molecules) RN - 0 (DC-specific ICAM-3 grabbing nonintegrin) RN - 0 (Lectins, C-Type) RN - 0 (Receptors, Cell Surface) RN - 0 (Receptors, Virus) RN - 0 (Recombinant Proteins) RN - 0 (Viral Envelope Proteins) RN - 0 (glycoprotein B, Simplexvirus) RN - 0 (glycoprotein gC, herpes simplex virus type 1) SB - IM MH - Cell Adhesion Molecules/genetics/*metabolism MH - Dendritic Cells/cytology/immunology/*virology MH - Herpes Simplex/*immunology/*transmission/virology MH - Herpesvirus 1, Human/metabolism/*pathogenicity/physiology MH - Herpesvirus 2, Human/metabolism/*pathogenicity MH - Humans MH - Lectins, C-Type/genetics/*metabolism MH - Monocytes/cytology MH - Receptors, Cell Surface/genetics/*metabolism MH - Receptors, Virus/metabolism MH - Recombinant Proteins/genetics/metabolism MH - Viral Envelope Proteins/metabolism EDAT- 2008/09/18 09:00 MHDA- 2008/10/28 09:00 CRDT- 2008/09/18 09:00 PHST- 2008/09/18 09:00 [pubmed] PHST- 2008/10/28 09:00 [medline] PHST- 2008/09/18 09:00 [entrez] AID - 10.1099/vir.0.2008/003129-0 [doi] PST - ppublish SO - J Gen Virol. 2008 Oct;89(Pt 10):2398-2409. doi: 10.1099/vir.0.2008/003129-0.