PMID- 18797815
OWN - NLM
STAT- MEDLINE
DCOM- 20081114
LR  - 20211020
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 153
IP  - 10
DP  - 2008
TI  - MHC-I-restricted epitopes conserved among variola and other related 
      orthopoxviruses are recognized by T cells 30 years after vaccination.
PG  - 1833-44
LID - 10.1007/s00705-008-0194-7 [doi]
AB  - It is many years since the general population has been vaccinated against 
      smallpox virus. Here, we report that human leukocyte antigen (HLA) class I 
      restricted T cell epitopes can be recognized more than 30 years after 
      vaccination. Using bioinformatic methods, we predicted 177 potential cytotoxic T 
      lymphocyte epitopes. Eight epitopes were confirmed to stimulate IFN-gamma release 
      by T cells in smallpox-vaccinated subjects. The epitopes were restricted by five 
      supertypes (HLA-A1, -A2, -A24 -A26 and -B44). Significant T cell responses were 
      detected against 8 of 45 peptides with an HLA class I affinity of K(D) less than 
      or equal to 5 nM, whereas no T cell responses were detected against 60 peptides 
      with an HLA affinity of K(D) more than 5 nM. All epitopes were fully conserved in 
      seven variola, vaccinia and cowpox strains. Knowledge of the long-term response 
      to smallpox vaccination may lead to a better understanding of poxvirus immunity 
      and may aid in the development of new improved vaccines and diagnostic tools.
FAU - Tang, S T
AU  - Tang ST
AD  - Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of 
      Denmark, Lyngby, Denmark.
FAU - Wang, M
AU  - Wang M
FAU - Lamberth, K
AU  - Lamberth K
FAU - Harndahl, M
AU  - Harndahl M
FAU - Dziegiel, M H
AU  - Dziegiel MH
FAU - Claesson, M H
AU  - Claesson MH
FAU - Buus, S
AU  - Buus S
FAU - Lund, O
AU  - Lund O
LA  - eng
GR  - N01 AI040083/AI/NIAID NIH HHS/United States
GR  - HHSN266200400083C/PHS HHS/United States
GR  - HHSN266200400025C/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080912
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Smallpox Vaccine)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Female
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Male
MH  - Middle Aged
MH  - Orthopoxvirus/*immunology
MH  - Smallpox Vaccine/*immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Time Factors
PMC - PMC2784598
MID - NIHMS75858
EDAT- 2008/09/18 09:00
MHDA- 2008/11/15 09:00
PMCR- 2009/11/27
CRDT- 2008/09/18 09:00
PHST- 2008/04/25 00:00 [received]
PHST- 2008/07/29 00:00 [accepted]
PHST- 2008/09/18 09:00 [pubmed]
PHST- 2008/11/15 09:00 [medline]
PHST- 2008/09/18 09:00 [entrez]
PHST- 2009/11/27 00:00 [pmc-release]
AID - 10.1007/s00705-008-0194-7 [doi]
PST - ppublish
SO  - Arch Virol. 2008;153(10):1833-44. doi: 10.1007/s00705-008-0194-7. Epub 2008 Sep 
      12.