PMID- 18798798
OWN - NLM
STAT- MEDLINE
DCOM- 20091112
LR  - 20090402
IS  - 1399-3038 (Electronic)
IS  - 0905-6157 (Linking)
VI  - 20
IP  - 2
DP  - 2009 Mar
TI  - Phenotypic analysis of circulating dendritic cells during the second half of 
      human gestation.
PG  - 119-25
LID - 10.1111/j.1399-3038.2008.00771.x [doi]
AB  - Dendritic cells (DCs) have been characterized as having an immature phenotype in 
      infants when compared with adults; but it is unclear whether the phenotype or 
      function of these populations changes during human intrauterine development. 
      Three-colour flow cytometry was used to phenotype fetal/neonatal circulating DCs 
      during the second half (>20-wk gestation) of pregnancy, (n = 34) and adults (n = 
      9). DCs were identified from peripheral blood mononuclear cells (PBMCs) or cord 
      blood mononuclear cells (CBMCs) as staining brightly for HLA-DR but negative for 
      T cell, B cell, monocyte, and NK cell lineage markers. The surface molecule of 
      interest was detected in a third colour. During gestation CD34, a marker of 
      immaturity was significantly higher, and CD4, a differentiation marker, was 
      significantly lower than adult levels. The percentage of CD11c+ cells did not 
      differ significantly at any age, although a trend to reduced intensity of 
      expression at earlier stages of gestation was observed. Significantly fewer DCs 
      expressed the IgG receptors CD32 and CD64 at all gestations. The percentage of 
      HLA-DR+/lin- cells expressing CD40 was lowest at 20-23 wks and was always 
      significantly lower on DCs from cord blood vs. adult blood. Similarly, the 
      percentage of CD86+ and CD54+ DCs was significantly lower than adults throughout 
      gestation. Thus, immaturity of cord blood DCs is likely to arise as a consequence 
      of decreased ability to take up antigen (at least via IgG-mediated mechanisms) 
      and reduced provision of co-stimulation.
FAU - Holloway, Judith A
AU  - Holloway JA
AD  - School of Medicine, University of Southampton, Southampton, UK. 
      j.holloway@soton.ac.uk
FAU - Thornton, Catherine A
AU  - Thornton CA
FAU - Diaper, Norma D
AU  - Diaper ND
FAU - Howe, David T
AU  - Howe DT
FAU - Warner, John O
AU  - Warner JO
LA  - eng
GR  - HL-61858/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080915
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (Antigens, Differentiation)
SB  - IM
MH  - Aborted Fetus/*immunology
MH  - Adult
MH  - Antigens, Differentiation/genetics/immunology/*metabolism
MH  - Blood Cells/cytology
MH  - Cell Differentiation/immunology
MH  - Dendritic Cells/cytology/immunology/*metabolism
MH  - Female
MH  - Fetal Development/*immunology
MH  - Humans
MH  - Immune System/*embryology/immunology
MH  - Immunophenotyping
MH  - Infant, Newborn
MH  - Pregnancy
MH  - Time Factors
MH  - Umbilical Veins/cytology
EDAT- 2008/09/19 09:00
MHDA- 2009/11/13 06:00
CRDT- 2008/09/19 09:00
PHST- 2008/09/19 09:00 [pubmed]
PHST- 2009/11/13 06:00 [medline]
PHST- 2008/09/19 09:00 [entrez]
AID - PAI771 [pii]
AID - 10.1111/j.1399-3038.2008.00771.x [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2009 Mar;20(2):119-25. doi: 
      10.1111/j.1399-3038.2008.00771.x. Epub 2008 Sep 15.