PMID- 18799129
OWN - NLM
STAT- MEDLINE
DCOM- 20081229
LR  - 20081208
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 378
IP  - 2
DP  - 2009 Jan 9
TI  - Regulation of matrix metalloproteinase-9 expression between gingival fibroblast 
      cells from old and young rats.
PG  - 152-6
LID - 10.1016/j.bbrc.2008.09.015 [doi]
AB  - Gingival fibroblast cells (rGF) from aged rats have an age-related decline in 
      proliferative capacity compared with young rats. We investigated G1 phase cell 
      cycle regulation and MMP-9 expression in both young and aged rGF. G1 cell cycle 
      protein levels and activity were significantly reduced in response to 
      interleukin-1beta (IL-1beta) stimulation with increasing in vitro age. Tumor 
      necrosis factor-alpha (TNF-alpha)-induced matrix metalloproteinase-9 (MMP-9) 
      expression was also decreased in aged rGF in comparison with young rGF. 
      Mutational analysis and gel shift assays demonstrated that the lower MMP-9 
      expression in aged rGF is associated with lower activities of transcription 
      factors NF-kappaB and AP-1. These results suggest that cell cycle dysregulation 
      and down-regulation of MMP-9 expression in rGF may play a role in gingival 
      remodeling during in vitro aging.
FAU - Kim, Su-Jung
AU  - Kim SJ
AD  - Department of Orthodontics, Kyung-Hee University School of Dentistry, Hoegi-Dong 
      1, Dongdaemoon-Ku, Seoul, Republic of Korea.
FAU - Chung, Yong-Koo
AU  - Chung YK
FAU - Chung, Tae-Wook
AU  - Chung TW
FAU - Kim, Jeong-Ran
AU  - Kim JR
FAU - Moon, Sung-Kwon
AU  - Moon SK
FAU - Kim, Cheorl-Ho
AU  - Kim CH
FAU - Park, Young-Guk
AU  - Park YG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20080915
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Interleukin-1beta)
RN  - 0 (NF-kappa B)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Age Factors
MH  - Aging/genetics/*metabolism/pathology
MH  - Animals
MH  - DNA/metabolism
MH  - DNA Mutational Analysis
MH  - Down-Regulation
MH  - Electrophoretic Mobility Shift Assay
MH  - Fibroblasts/drug effects/enzymology/pathology
MH  - G1 Phase/drug effects
MH  - *Gene Expression Regulation, Enzymologic
MH  - Gingiva/drug effects/*enzymology/pathology
MH  - Interleukin-1beta/pharmacology
MH  - Male
MH  - Matrix Metalloproteinase 9/*genetics
MH  - NF-kappa B/metabolism
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription Factor AP-1/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2008/09/19 09:00
MHDA- 2008/12/30 09:00
CRDT- 2008/09/19 09:00
PHST- 2008/08/29 00:00 [received]
PHST- 2008/09/08 00:00 [accepted]
PHST- 2008/09/19 09:00 [pubmed]
PHST- 2008/12/30 09:00 [medline]
PHST- 2008/09/19 09:00 [entrez]
AID - S0006-291X(08)01773-7 [pii]
AID - 10.1016/j.bbrc.2008.09.015 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Jan 9;378(2):152-6. doi: 
      10.1016/j.bbrc.2008.09.015. Epub 2008 Sep 15.