PMID- 1879972
OWN - NLM
STAT- MEDLINE
DCOM- 19911002
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 49
IP  - 2
DP  - 1991 Sep 9
TI  - Subcellular localization, redistribution and photobleaching of sulfonated 
      aluminum phthalocyanines in a human melanoma cell line.
PG  - 290-5
AB  - Intracellular localization, intracellular translocation and photobleaching 
      following non-lethal laser microirradiation of the fluorescing derivatives of 
      sulfonated aluminum phthalocyanines (Al-PcSn, n = 1-4) in a human melanoma cell 
      line (LOX) were studied by means of confocal laser scanning microscopy (LSM) and 
      image processing. Use of confocal microscopy allowed 3-dimensional information to 
      be obtained. Both Al-PcS1 and Al-PcS2 localized diffusely in the cytoplasm of the 
      cells, while Al-PcS3 and Al-PcS4 exhibited a granular pattern in the extranuclear 
      fraction of the cells. None of the Al-PcSn family was observed in the nuclei of 
      the cells except that a small fraction of fluorescence was occasionally detected 
      in nuclei of some cells treated with Al-PcS1 and Al-PcS2. Furthermore, exactly 
      the same granular localization patterns and positions in the same cells were 
      found after incubation initially with Al-PcS3 (or Al-PcS4) followed by acridine 
      orange (AO) which emits red fluorescence from lysosomes of cells. Thus, the 
      granular fluorescence of Al-PcS3 and Al-PcS4 is confined to the lysosomes of the 
      LOX cells. Non-lethal laser exposure of cells incubated with high concentrations 
      of the 2 dyes resulted in a translocation of the dyes from the lysosomes to the 
      whole cytoplasm and an increase in total intracellular fluorescence intensity. 
      Finally, a small fraction of the dyes localized into the nuclei of the cells. The 
      laser exposure of cells incubated with low concentrations of the lysosomally 
      localized dyes resulted in an increase in the intracellular fluorescence 
      intensity with no translocation of the dyes. Under all conditions, high laser 
      exposure resulted in a decrease in the total intracellular fluorescence 
      intensity.
FAU - Peng, Q
AU  - Peng Q
AD  - Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, 
      Norway.
FAU - Farrants, G W
AU  - Farrants GW
FAU - Madslien, K
AU  - Madslien K
FAU - Bommer, J C
AU  - Bommer JC
FAU - Moan, J
AU  - Moan J
FAU - Danielsen, H E
AU  - Danielsen HE
FAU - Nesland, J M
AU  - Nesland JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Indoles)
RN  - 0 (Organometallic Compounds)
RN  - 47822-79-7 (aluminum phthalocyanine)
SB  - IM
MH  - Cell Nucleus/chemistry/*metabolism
MH  - Humans
MH  - Indoles/analysis/*metabolism
MH  - Lasers
MH  - Melanoma/chemistry/*metabolism
MH  - Microscopy, Fluorescence/methods
MH  - Organometallic Compounds/analysis/*metabolism
MH  - Photomicrography
MH  - Tumor Cells, Cultured/chemistry/metabolism
EDAT- 1991/09/09 00:00
MHDA- 1991/09/09 00:01
CRDT- 1991/09/09 00:00
PHST- 1991/09/09 00:00 [pubmed]
PHST- 1991/09/09 00:01 [medline]
PHST- 1991/09/09 00:00 [entrez]
AID - 10.1002/ijc.2910490225 [doi]
PST - ppublish
SO  - Int J Cancer. 1991 Sep 9;49(2):290-5. doi: 10.1002/ijc.2910490225.