PMID- 18799930
OWN - NLM
STAT- MEDLINE
DCOM- 20090413
LR  - 20240308
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 26
IP  - 6
DP  - 2008 Dec 31
TI  - Rosmarinic acid down-regulates the LPS-induced production of monocyte 
      chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1alpha 
      (MIP-1alpha) via the MAPK pathway in bone-marrow derived dendritic cells.
PG  - 583-9
AB  - In the present study, we investigated whether rosmarinic acid, which has been 
      suggested to exhibit anti-inflammatory properties, can suppress the expressions 
      of monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory 
      protein-1alpha (MIP-1alpha) via the MAPK pathway in LPS-stimulated bone 
      marrow-derived dendritic cells (BMDCs) in the presence of GM-CSF and IL-4 in 
      media. The effects of rosmarinic acid were investigated in BMDCs with respect to 
      the following; cytotoxicity, surface molecule expression, dextran-FITC uptake, 
      cell migration, chemokine gene expression, and the MAPK signaling pathway. 
      Rosmarinic acid was found to significantly inhibit the expressions of CD80, CD86, 
      MHC class I, and MHC class II in LPS-stimulated mature BMDCs, and rosmarinic 
      acid-treated BMDCs were found to be highly efficient with regards to antigen 
      capture via mannose receptor-mediated endocytosis. In addition, rosmarinic acid 
      reduced cell migration by inducing the expression of a specific chemokine 
      receptor on LPS-induced mature BMDCs. Rosmarinic acid also significantly reduced 
      the expressions of MCP-1 and MIP-lalpha induced by LPS in BMDCs and inhibited 
      LPS-induced activation of MAPK and the nuclear translocation of NF-kappaB. These 
      findings broaden current perspectives concerning our understanding of the 
      immunopharmacological functions of rosmarinic acid, and have ramifications that 
      concern the development of therapeutic drugs for the treatment of DC-related 
      acute and chronic diseases.
FAU - Kim, Hyung Keun
AU  - Kim HK
AD  - Department of Orthopaedics, Chonnam National University, Hwasun Hospital, Chonnam 
      519-809, Korea.
FAU - Lee, Jae Joon
AU  - Lee JJ
FAU - Lee, Jun Sik
AU  - Lee JS
FAU - Park, Yeong-Min
AU  - Park YM
FAU - Yoon, Taek Rim
AU  - Yoon TR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (Ccl3 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Cinnamates)
RN  - 0 (Depsides)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Transcription Factor RelA)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/drug effects/metabolism
MH  - Chemokine CCL2/*biosynthesis
MH  - Chemokine CCL3/*biosynthesis
MH  - Cinnamates/*pharmacology
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Depsides/*pharmacology
MH  - Down-Regulation
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/*physiology
MH  - Protein Transport/drug effects
MH  - Transcription Factor RelA/metabolism
MH  - Rosmarinic Acid
EDAT- 2008/09/19 09:00
MHDA- 2009/04/14 09:00
CRDT- 2008/09/19 09:00
PHST- 2008/09/19 09:00 [pubmed]
PHST- 2009/04/14 09:00 [medline]
PHST- 2008/09/19 09:00 [entrez]
AID - S1016-8478(23)25236-X [pii]
PST - ppublish
SO  - Mol Cells. 2008 Dec 31;26(6):583-9.