PMID- 18800682
OWN - NLM
STAT- MEDLINE
DCOM- 20110616
LR  - 20080919
IS  - 2096-7993 (Print)
IS  - 2096-7993 (Linking)
VI  - 22
IP  - 12
DP  - 2008 Jun
TI  - [Detection of Skp2 mRNA gene expression using fluorogenic probe quantitative 
      RT-PCR method in laryngeal squamous cell carcinoma].
PG  - 547-50
AB  - OBJECTIVE: The current study was designed to examine the expression of Skp2 gene 
      in laryngeal squamous cell carcinoma (LSCC) and to investigate the role of Skp2 
      gene in tumorigenesis and progression of LSCC. METHOD: FQ-PCR method was used to 
      examined the expression of Skp2 gene in 40 LSCC and 10 normal laryngeal mucosa 
      tissues, and relationship between its expression and clinical biological factors 
      of patients with LSCC was analyzed. RESULT: The median copy number of Skp2 mRNA 
      expression in LSCC was 6622.54 copy/microg RNA, the median copy number of Skp2 
      mRNA expression in normal laryngeal mucosa tissues was 0 copy/microg RNA, there 
      was a very significant difference between them (P < 0.01); The positive rate of 
      Skp2 mRNA expression in LSCC and adjacent normal laryngeal tissue were 50%, 0, 
      respectively (P < 0.01). The median copy number of Skp2 RNA expression in LSCC 
      with cervical lymph node metastasis was 617138.4 copy/microg RNA, the median copy 
      number of Skp2 mRNA expression in LSCC without cervical lymph node metastasis was 
      0 copy/microg RNA, there was a very significant difference between them (P < 
      0.05); The positive rate of Skp2 mRNA expression in LSCC with and without 
      cervical lymph node metastasis were 100.00%, 35.48%, respectively (P < 0.01). 
      CONCLUSION: Skp2 gene might have relation with the cervical lymph node metastasis 
      of LSCC. FQ-PCR is an accurate assay to detecting expression of Skp2 mRNA in 
      patient with LSCC. The level of Skp2 mRNA expression might be a new and more 
      accurate marker, and it can be used to predict cervical lymph node metastasis of 
      LSCC.
FAU - Guo, Jianfeng
AU  - Guo J
AD  - Department of Otolaryngology, Dongguan People's Hospital, Dongguan, 523018, 
      China.
FAU - Chen, Fujin
AU  - Chen F
FAU - Guan, Zhiwei
AU  - Guan Z
FAU - Wu, Qili
AU  - Wu Q
FAU - Zeng, Chaohui
AU  - Zeng C
FAU - Chen, Yanfeng
AU  - Chen Y
FAU - Li, Qiuli
AU  - Li Q
FAU - Ou-Yang, Dian
AU  - Ou-Yang D
FAU - Hu, Bin
AU  - Hu B
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical 
      otorhinolaryngology, head, and neck surgery
JID - 101303164
RN  - 0 (RNA, Messenger)
RN  - 0 (S-Phase Kinase-Associated Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Messenger/genetics
MH  - S-Phase Kinase-Associated Proteins/genetics/*metabolism
EDAT- 2008/09/20 09:00
MHDA- 2011/06/17 06:00
CRDT- 2008/09/20 09:00
PHST- 2008/09/20 09:00 [pubmed]
PHST- 2011/06/17 06:00 [medline]
PHST- 2008/09/20 09:00 [entrez]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2008 Jun;22(12):547-50.