PMID- 18800984
OWN - NLM
STAT- MEDLINE
DCOM- 20100125
LR  - 20191210
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 128
IP  - 1 Suppl
DP  - 2009 Sep
TI  - Uptake and presentation of exogenous antigen and presentation of endogenously 
      produced antigen by skin dendritic cells represent equivalent pathways for the 
      priming of cellular immune responses following biolistic DNA immunization.
PG  - e193-205
LID - 10.1111/j.1365-2567.2008.02947.x [doi]
AB  - Gene gun-mediated biolistic DNA vaccination with beta-galactosidase 
      (betaGal)-encoding plasmid vectors efficiently modulated antigen-induced immune 
      responses in an animal model of type I allergy, including the inhibition of 
      immunoglobulin E (IgE) production. Here we show that CD4(+) as well as CD8(+) T 
      cells from mice biolistically transfected with a plasmid encoding betaGal under 
      the control of the fascin promoter (pFascin-betaGal) are capable of inhibiting 
      betaGal-specific IgE production after adoptive transfer into naive recipients. 
      Moreover, suppression of IgE production was dependent on interferon (IFN)-gamma. 
      To analyse the modalities of activation of CD4(+) and CD8(+) T cells regarding 
      the localization of antigen synthesis following gene gun-mediated DNA 
      immunization, we used the fascin promoter and the keratin 5 promoter 
      (pK5-betaGal) to direct betaGal production mainly to dendritic cells (DCs) and to 
      keratinocytes, respectively. Gene gun-mediated DNA immunization with each vector 
      induced considerable activation of betaGal-specific CD8(+) cytotoxic T cells. 
      Cytokine production by re-stimulated CD4(+) T cells in draining lymph nodes and 
      immunoglobulin isotype profiles in sera of immunized mice indicated that 
      immunization with pFascin-betaGal induced a T helper type 1 (Th1)-biased immune 
      response, whereas immunization with pK5-betaGal generated a mixed Th1/Th2 immune 
      response. Nevertheless, DNA vaccination with pFascin-betaGal and pK5-betaGal, 
      respectively, efficiently inhibited specific IgE production in the mouse model of 
      type I allergy. In conclusion, our data show that uptake of exogenous antigen 
      produced by keratinocytes and its presentation by untransfected DCs as well as 
      the presentation of antigen synthesized endogenously in DCs represent equivalent 
      pathways for efficient priming of cellular immune responses.
FAU - Sudowe, Stephan
AU  - Sudowe S
AD  - Department of Dermatology, Johannes Gutenberg-University Mainz, Mainz, Germany. 
      sudowe@mail.uni-mainz.de
FAU - Dominitzki, Sabine
AU  - Dominitzki S
FAU - Montermann, Evelyn
AU  - Montermann E
FAU - Bros, Matthias
AU  - Bros M
FAU - Grabbe, Stephan
AU  - Grabbe S
FAU - Reske-Kunz, Angelika B
AU  - Reske-Kunz AB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080917
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Keratin-15)
RN  - 0 (Keratin-5)
RN  - 0 (Krt15 protein, mouse)
RN  - 0 (Vaccines, DNA)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - *Antigen Presentation
MH  - *Biolistics
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cross-Priming/genetics/immunology
MH  - Cytotoxicity, Immunologic/genetics/immunology
MH  - Female
MH  - Genetic Vectors/immunology/metabolism
MH  - Hypersensitivity/*therapy
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Interferon-gamma/genetics/immunology/metabolism
MH  - Interleukin-12 Subunit p40/genetics/immunology/metabolism
MH  - Keratin-15
MH  - Keratin-5/immunology/metabolism
MH  - Keratinocytes/immunology
MH  - Langerhans Cells/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Promoter Regions, Genetic/immunology
MH  - T-Lymphocytes, Helper-Inducer/enzymology/immunology
MH  - Vaccination/*methods
MH  - Vaccines, DNA/*administration & dosage/immunology
MH  - beta-Galactosidase/genetics
PMC - PMC2753911
EDAT- 2008/09/20 09:00
MHDA- 2010/01/26 06:00
PMCR- 2010/09/01
CRDT- 2008/09/20 09:00
PHST- 2008/09/20 09:00 [pubmed]
PHST- 2010/01/26 06:00 [medline]
PHST- 2008/09/20 09:00 [entrez]
PHST- 2010/09/01 00:00 [pmc-release]
AID - IMM2947 [pii]
AID - 10.1111/j.1365-2567.2008.02947.x [doi]
PST - ppublish
SO  - Immunology. 2009 Sep;128(1 Suppl):e193-205. doi: 
      10.1111/j.1365-2567.2008.02947.x. Epub 2008 Sep 17.