PMID- 18801463
OWN - NLM
STAT- MEDLINE
DCOM- 20090423
LR  - 20231213
IS  - 1567-5769 (Print)
IS  - 1567-5769 (Linking)
VI  - 8
IP  - 13-14
DP  - 2008 Dec 20
TI  - Signaling pathway for TNF-alpha-induced MMP-9 expression: mediation through p38 
      MAP kinase, and inhibition by anti-cancer molecule magnolol in human urinary 
      bladder cancer 5637 cells.
PG  - 1821-6
LID - 10.1016/j.intimp.2008.08.018 [doi]
AB  - We investigated the molecular mechanisms involved in the expression of matrix 
      metalloproteinase-9 (MMP-9) and the inhibition of MMP expression by magnolol in 
      5637 human urinary bladder cancer cells. Tumor necrosis factor-alpha (TNF-alpha) 
      stimulated the secretion of MMP-9 in 5637 cells, as shown by zymography and 
      promoter assay. The transcription factor nuclear factor kappaB (NF-kappaB) 
      binding site was identified by gel-shift assay to be a cis-element for TNF-alpha 
      activation of the MMP-9 promoter. Our results also demonstrated that TNF-alpha 
      stimulates MMP-9 expression via the p38 MAP kinase signaling pathway in 5637 
      cells. Moreover, p38 MAP kinase-mediated MMP-9 gene regulation in response to 
      TNF-alpha is involved in the NF-kappaB response element in 5637 cells. In 
      addition, magnolol inhibited TNF-alpha-induced expression of the MMP-9, as 
      determined by zymography and immunoblot, in 5637 cells. The TNF-alpha-induced 
      invasion and migration of cells was inhibited by magnolol, as assessed by a 
      modified boyden chamber and wound-healing assays, respectively. Finally, magnolol 
      blocked MMP-9 expression, at least in part, by decreasing the binding of 
      transcription factor NF-kappaB to DNA. In conclusion, TNF-alpha induced MMP-9 
      expression in 5637 cells by activating the transcription factor NF-kappaB, which 
      is involved in the p38 MAP kinase-mediated control of MMP-9 regulation. Magnolol 
      inhibited MMP-9 expression through the transcription factor NF-kappaB in 
      TNF-alpha-induced 5637 cells.
FAU - Lee, Se-Jung
AU  - Lee SJ
AD  - Department of Food and Biotechnology, Chungju National University, Chungju, 
      Chungbuk 380-702, South Korea.
FAU - Park, Sung-Soo
AU  - Park SS
FAU - Lee, Ung-Soo
AU  - Lee US
FAU - Kim, Wun-Jae
AU  - Kim WJ
FAU - Moon, Sung-Kwon
AU  - Moon SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080916
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Imidazoles)
RN  - 0 (Lignans)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Pyridines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 001E35HGVF (magnolol)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Biphenyl Compounds/*pharmacology
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Enzyme Inhibitors/*pharmacology
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - Lignans/*pharmacology
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Protein Serine-Threonine Kinases/drug effects/metabolism
MH  - Pyridines/pharmacology
MH  - Signal Transduction/drug effects
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Urinary Bladder Neoplasms/*enzymology
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
MH  - NF-kappaB-Inducing Kinase
EDAT- 2008/09/20 09:00
MHDA- 2009/04/25 09:00
CRDT- 2008/09/20 09:00
PHST- 2008/04/24 00:00 [received]
PHST- 2008/08/26 00:00 [revised]
PHST- 2008/08/26 00:00 [accepted]
PHST- 2008/09/20 09:00 [pubmed]
PHST- 2009/04/25 09:00 [medline]
PHST- 2008/09/20 09:00 [entrez]
AID - S1567-5769(08)00269-5 [pii]
AID - 10.1016/j.intimp.2008.08.018 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2008 Dec 20;8(13-14):1821-6. doi: 
      10.1016/j.intimp.2008.08.018. Epub 2008 Sep 16.