PMID- 18804087
OWN - NLM
STAT- MEDLINE
DCOM- 20081204
LR  - 20081030
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 383
IP  - 2
DP  - 2008 Dec 15
TI  - A gas chromatographic method for quantification of detergents frequently used in 
      membrane protein structural studies.
PG  - 326-8
LID - 10.1016/j.ab.2008.08.028 [doi]
AB  - A novel and reliable gas chromatography-flame ionization detection (GC-FID) 
      method that can separate and quantify detergents frequently used in membrane 
      protein structural studies has been developed. Different detergents were 
      identified through FID peaks with different retention times. A quadratic 
      regression curve was found to fit the integrated FID peak area against different 
      detergent concentrations. Detergents can be quantified as low as the nanogram 
      level: lauryl-dimethylamine-N-oxide (LDAO), 5 ng; dodecyl maltoside (DDM), 10 ng; 
      and dodecyl phosphocholine (DPC), 50 ng. This method can be applied directly to 
      measure detergent concentration and molar ratio of membrane protein to detergents 
      during membrane protein extraction, purification, concentration, and 
      crystallization.
FAU - Shi, Chaowei
AU  - Shi C
AD  - National Laboratory for Physical Science at Microscale, School of Life Science, 
      University of Science and Technology of China, Hefei, Anhui 230026, People's 
      Republic of China.
FAU - Shao, Wei
AU  - Shao W
FAU - Xiong, Ying
AU  - Xiong Y
FAU - Tian, Changlin
AU  - Tian C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080903
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Detergents)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Chromatography, Gas/*methods
MH  - Detergents/*analysis/isolation & purification
MH  - Flame Ionization
MH  - Membrane Proteins/*chemistry
EDAT- 2008/09/23 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/09/23 09:00
PHST- 2008/06/10 00:00 [received]
PHST- 2008/08/25 00:00 [revised]
PHST- 2008/08/27 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
AID - S0003-2697(08)00583-6 [pii]
AID - 10.1016/j.ab.2008.08.028 [doi]
PST - ppublish
SO  - Anal Biochem. 2008 Dec 15;383(2):326-8. doi: 10.1016/j.ab.2008.08.028. Epub 2008 
      Sep 3.