PMID- 18804108
OWN - NLM
STAT- MEDLINE
DCOM- 20081210
LR  - 20120409
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 582
IP  - 23-24
DP  - 2008 Oct 15
TI  - GO6976 prevents TNF-alpha-induced suppression of adiponectin expression in 3T3-L1 
      adipocytes: putative involvement of protein kinase C.
PG  - 3473-8
LID - 10.1016/j.febslet.2008.09.012 [doi]
AB  - Adiponectin, one of the adipokines secreted by adipocytes, possesses insulin 
      sensitizing and anti-atherosclerotic properties. Tumor necrosis factor-alpha 
      (TNF-alpha) is known to suppress the expression and secretion of adiponectin in 
      adipocytes; however, the underlying mechanism remains poorly understood. Here, we 
      demonstrate that GO6976 (a selective inhibitor of conventional protein kinase C 
      (PKC)) prevents TNF-alpha-induced suppression of adiponectin secretion and 
      expression in fully differentiated 3T3-L1 adipocytes, accompanied by attenuation 
      of c-Jun N-terminal kinase (JNK) activation. Additionally, the transcriptional 
      activity of peroxisome proliferator-activated receptor-gamma (PPARg) (a strong 
      inducer of adiponectin) on the adiponectin promoter was inhibited in a PKC 
      isoform-specific manner. These results raise the possibility that PKC is involved 
      in TNF-alpha-induced suppression of adiponectin in 3T3-L1 adipocytes.
FAU - Lim, Joong-Yeon
AU  - Lim JY
AD  - Division of Intractable Diseases, Center for Biomedical Sciences, National 
      Institute of Health, 194 Tongillo, Eunpyeong-gu, Seoul 122-701, Republic of 
      Korea.
FAU - Kim, Won Ho
AU  - Kim WH
FAU - Park, Sang Ick
AU  - Park SI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080917
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Adiponectin)
RN  - 0 (Adipoq protein, mouse)
RN  - 0 (Carbazoles)
RN  - 0 (Isoenzymes)
RN  - 0 (PPAR gamma)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 136194-77-9 (Go 6976)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*drug effects/metabolism
MH  - Adiponectin/antagonists & inhibitors/biosynthesis/genetics
MH  - Animals
MH  - Carbazoles/*pharmacology
MH  - Isoenzymes/antagonists & inhibitors
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Mice
MH  - PPAR gamma/antagonists & inhibitors/metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Kinase C/*antagonists & inhibitors
MH  - Protein Kinase Inhibitors/*pharmacology
MH  - Transcription, Genetic
MH  - Tumor Necrosis Factor-alpha/pharmacology/physiology
EDAT- 2008/09/23 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/09/23 09:00
PHST- 2008/06/10 00:00 [received]
PHST- 2008/08/19 00:00 [revised]
PHST- 2008/09/09 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
AID - S0014-5793(08)00745-X [pii]
AID - 10.1016/j.febslet.2008.09.012 [doi]
PST - ppublish
SO  - FEBS Lett. 2008 Oct 15;582(23-24):3473-8. doi: 10.1016/j.febslet.2008.09.012. 
      Epub 2008 Sep 17.