PMID- 18804197
OWN - NLM
STAT- MEDLINE
DCOM- 20090731
LR  - 20190508
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 54
IP  - 1
DP  - 2009 Jan
TI  - Potential biomarkers of human salivary function: a modified proteomic approach.
PG  - 91-100
LID - 10.1016/j.archoralbio.2008.08.007 [doi]
AB  - OBJECTIVE: In previous studies, we defined groups of subjects with opposite 
      salivary function. Group membership was associated with clinically relevant 
      outcomes. High aggregation-adherence (HAA) groups showed lower levels of caries, 
      supragingival plaque, total streptococci, and Tannerella forsythensis than low 
      high aggregation-adherence (LAA) groups. In this study, we used a proteomic 
      approach to search for biomarkers which could be useful as risk indicators for 
      those outcomes. DESIGN: Clarified resting whole saliva from each of 41 HAA and 
      LAA subjects was separated by preparative isoelectric focusing. Fractions showing 
      the most distinctive protein profiles were pooled into four sets (pI 3-3.5, pI 
      4-4.7, pI 5.7-7.7, pI 10-11.5). Each pool then was compared by SDS-PAGE. Image 
      analysis software was used to quantify matched bands. Partial least squares 
      analysis (PLS) was used to determine which of the 65 bands from all four pools 
      were the best predictors of group membership, caries, total plaque, total 
      streptococci, and T. forsythensis counts. Those bands were identified by mass 
      spectroscopy (MS-MS). RESULTS: Two bands consistently were strong predictors in 
      separate PLS analyses of each outcome variable. In follow-up univariate analyses, 
      those bands showed the strongest significant differences between the HAA and LAA 
      groups. They also showed significant inverse correlations with caries and all the 
      microbiological variables. MSMS identified those bands as statherin, and a 
      truncated cystatin S missing the first eight N-terminal amino acids. CONCLUSIONS: 
      Levels of statherin and truncated cystatin S may be potential risk indicators for 
      the development of caries and other oral diseases.
FAU - Rudney, J D
AU  - Rudney JD
AD  - Department of Diagnostic and Biological Sciences, School of Dentistry, 17-252 
      Moos Tower, 515 Delaware St. SE, University of Minnesota, Minneapolis, MN 55455, 
      USA. jrudney@tc.umn.edu
FAU - Staikov, R K
AU  - Staikov RK
FAU - Johnson, J D
AU  - Johnson JD
LA  - eng
GR  - R01 DE007233-15/DE/NIDCR NIH HHS/United States
GR  - 2 R01 DE 07233/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080918
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Biomarkers)
RN  - 0 (STATH protein, human)
RN  - 0 (Salivary Cystatins)
RN  - 0 (Salivary Proteins and Peptides)
SB  - IM
MH  - Bacterial Adhesion/physiology
MH  - Biofilms
MH  - Biomarkers/analysis
MH  - Blotting, Western
MH  - Colony Count, Microbial
MH  - Dental Caries/*microbiology
MH  - Electrophoresis, Gel, Two-Dimensional/methods
MH  - Female
MH  - Humans
MH  - Male
MH  - Proteomics/methods
MH  - Risk Factors
MH  - Saliva/*chemistry/microbiology/physiology
MH  - Salivary Cystatins/*analysis
MH  - Salivary Proteins and Peptides/*analysis
PMC - PMC2633945
MID - NIHMS89364
EDAT- 2008/09/23 09:00
MHDA- 2009/08/01 09:00
PMCR- 2010/01/01
CRDT- 2008/09/23 09:00
PHST- 2007/09/06 00:00 [received]
PHST- 2008/08/14 00:00 [revised]
PHST- 2008/08/21 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2009/08/01 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
PHST- 2010/01/01 00:00 [pmc-release]
AID - S0003-9969(08)00233-1 [pii]
AID - 10.1016/j.archoralbio.2008.08.007 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2009 Jan;54(1):91-100. doi: 10.1016/j.archoralbio.2008.08.007. 
      Epub 2008 Sep 18.