PMID- 18804211
OWN - NLM
STAT- MEDLINE
DCOM- 20090311
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1213
IP  - 1
DP  - 2008 Dec 5
TI  - Analysis of indandione anticoagulant rodenticides in animal liver by eluent 
      generator reagent free ion chromatography coupled with electrospray mass 
      spectrometry.
PG  - 77-82
LID - 10.1016/j.chroma.2008.08.100 [doi]
AB  - A novel analytical method has been developed for simultaneous determination of 
      four indandione anticoagulant rodenticides (diphacinone, chlorophacinone, pindone 
      and valone) in animal liver tissues by eluent generator reagent free ion 
      chromatography coupled with electrospray ionization mass spectrometry 
      (RFIC-ESI-MS). After the rodenticides were extracted from homogenized animal 
      liver tissues with methanol-acetonitrile (10/90, v/v), the extracts were 
      subjected to a solid-phase extraction (SPE) process using Oasis HLB cartridges. 
      The IC separation was carried out on an IonPac AS11 analytical column (250 mm x 
      4.0 mm) using 10% methanol in a gradient of KOH solution at a constant flow rate 
      of 1.0 mL/min. The objective compounds were ionized by negative ion pneumatically 
      assisted electrospray and detected in the selected ion monitoring (SIM) mode. 
      Warfarin was applied as an internal standard (IS) for the compensation of the 
      losses in the course of sample processing and the sensitivity drift of the 
      detector, linear calibration functions were calculated for all analytes. The 
      relative average recoveries of the objective compounds spiked in animal liver 
      tissues were between 83.4 and 104.9%. The limits of quantification (LOQs) were 
      0.2-1.0 ng/g for them. Within-day and day-to-day relative standard deviations 
      (RSDs) were less than 10.4 and 13.3%, respectively. It was confirmed that this 
      method could be used in a toxicological analysis. The coupling of IC to MS 
      provided a new analytical tool to the analysts faced with the requirement of 
      separating and analyzing indandione rodenticides in animal livers.
FAU - Jin, Mi-cong
AU  - Jin MC
AD  - Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou, Zhejiang 
      Province 310028, China.
FAU - Chen, Xiao-hong
AU  - Chen XH
FAU - Ye, Ming-li
AU  - Ye ML
FAU - Zhu, Yan
AU  - Zhu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080903
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (1,2-indanedione)
RN  - 0 (Indans)
RN  - 0 (Rodenticides)
RN  - 2KFI1XBH7G (pindone)
RN  - 34Y6E0063Y (chlorophacinone)
RN  - 54CA01C6JX (diphenadione)
RN  - 5M7Y6274ZE (Phenindione)
SB  - IM
MH  - Animals
MH  - Chromatography, Ion Exchange/*methods
MH  - Dogs
MH  - Ducks
MH  - Indans/analysis
MH  - Liver/*chemistry
MH  - Phenindione/analogs & derivatives/analysis
MH  - Reproducibility of Results
MH  - Rodenticides/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Swine
EDAT- 2008/09/23 09:00
MHDA- 2009/03/12 09:00
CRDT- 2008/09/23 09:00
PHST- 2008/05/31 00:00 [received]
PHST- 2008/08/26 00:00 [revised]
PHST- 2008/08/29 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2009/03/12 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
AID - S0021-9673(08)01497-0 [pii]
AID - 10.1016/j.chroma.2008.08.100 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Dec 5;1213(1):77-82. doi: 10.1016/j.chroma.2008.08.100. Epub 
      2008 Sep 3.