PMID- 18804253
OWN - NLM
STAT- MEDLINE
DCOM- 20081223
LR  - 20181113
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 381
IP  - 1
DP  - 2008 Nov 10
TI  - Macropinocytosis and cytoskeleton contribute to dendritic cell-mediated HIV-1 
      transmission to CD4+ T cells.
PG  - 143-54
LID - 10.1016/j.virol.2008.08.028 [doi]
AB  - Dendritic cells (DCs) are among the first immune cells to encounter HIV-1 at the 
      initial infection. DCs efficiently transfer HIV-1 to CD4+ T cells via infectious 
      or virological synapses formed between DCs and T cells. Retroviruses exploit the 
      cytoskeletal network to facilitate viral infection and dissemination; however, 
      the role of the cytoskeleton in DC-mediated HIV-1 transmission is unknown. Here, 
      we report that intact cytoskeleton is essential for DC-mediated HIV-1 
      transmission to CD4+ T cells. We found that macropinocytosis of HIV-1 contributes 
      to DC-mediated HIV-1 endocytosis and transmission. Blocking HIV-1 
      macropinocytosis and disrupting actin or microtubules in DCs with specific 
      inhibitors significantly prevented DC-mediated HIV-1 trans-infection of CD4+ T 
      cells. Altered HIV-1 trafficking and impaired formation of virological synapses 
      primarily accounted for the inhibition of viral transmission by cytoskeletal 
      inhibitors. Our results provide new insights into the mechanisms underlying 
      DC-mediated HIV-1 transmission to CD4+ T cells via the cytoskeletal network.
FAU - Wang, Jian-Hua
AU  - Wang JH
AD  - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 
      8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
FAU - Wells, Clive
AU  - Wells C
FAU - Wu, Li
AU  - Wu L
LA  - eng
GR  - 1S10RR022412-01/RR/NCRR NIH HHS/United States
GR  - AI068493/AI/NIAID NIH HHS/United States
GR  - S10 RR022412/RR/NCRR NIH HHS/United States
GR  - R01 AI068493-03/AI/NIAID NIH HHS/United States
GR  - R01 AI068493/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080918
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Actins)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Tubulin Modulators)
RN  - SH1WY3R615 (Nocodazole)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Actins/metabolism
MH  - CD4-Positive T-Lymphocytes/metabolism/*virology
MH  - Cells, Cultured
MH  - Cytoskeleton/*metabolism
MH  - Dendritic Cells/drug effects/metabolism/ultrastructure/*virology
MH  - Dimethyl Sulfoxide/pharmacology
MH  - Free Radical Scavengers/pharmacology
MH  - HIV Infections/*transmission/virology
MH  - HIV-1/*physiology
MH  - Humans
MH  - Nocodazole/pharmacology
MH  - Pinocytosis/*physiology
MH  - Tubulin Modulators/pharmacology
PMC - PMC2575140
MID - NIHMS65955
EDAT- 2008/09/23 09:00
MHDA- 2008/12/24 09:00
PMCR- 2009/11/10
CRDT- 2008/09/23 09:00
PHST- 2008/07/11 00:00 [received]
PHST- 2008/08/12 00:00 [revised]
PHST- 2008/08/15 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2008/12/24 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
PHST- 2009/11/10 00:00 [pmc-release]
AID - S0042-6822(08)00550-3 [pii]
AID - 10.1016/j.virol.2008.08.028 [doi]
PST - ppublish
SO  - Virology. 2008 Nov 10;381(1):143-54. doi: 10.1016/j.virol.2008.08.028. Epub 2008 
      Sep 18.