PMID- 18804617
OWN - NLM
STAT- MEDLINE
DCOM- 20081103
LR  - 20161124
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 77
IP  - 1
DP  - 2008 Oct 19
TI  - Fluorescence resonance energy transfer between acridine orange and rhodamine 6G 
      and its analytical application for vitamin B12 with flow-injection laser-induced 
      fluorescence detection.
PG  - 176-81
LID - 10.1016/j.talanta.2008.06.022 [doi]
AB  - By coupling flow-injection with laser-induced fluorescence detection, a setup was 
      developed and a novel method combining fluorescence resonance energy transfer 
      (FRET) and flow-injection analysis (FIA) was proposed for the determination of 
      vitamin B(12) (VB(12)) based on its fluorescence quenching on the system of 
      acridine orange (AO)/rhodamine 6G (R6G). The effective energy transfer could 
      occur between AO and R6G in the dodecyl benzene sodium sulfonate (DBS) while 
      454nm argon laser was used as the excitation source, and as a result, the 
      fluorescence emission of R6G has been increased significantly. It was found that 
      the fluorescence of the above system could be sharply diminished by VB(12). By 
      using the mixed solution AO-R6G-DBS and the same solution containing VB(12) as 
      the carrier and sample, respectively, a series of negative peaks which could be 
      applied for the quantification of VB(12) were obtained. The detection limit for 
      VB(12) was 1.65x10(-6)mol/L. The linear range for determining VB(12) was 4x10(-4) 
      to 2x10(-6)mol/L (correlation coefficient, r=0.9923). The method was applied to 
      measure VB(12) injections with satisfactory results.
FAU - Xu, Hao
AU  - Xu H
AD  - College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, PR 
      China.
FAU - Li, Ying
AU  - Li Y
FAU - Liu, Chunmei
AU  - Liu C
FAU - Wu, Qiongshui
AU  - Wu Q
FAU - Zhao, Yu
AU  - Zhao Y
FAU - Lu, Li
AU  - Lu L
FAU - Tang, Hongwu
AU  - Tang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080625
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Rhodamines)
RN  - 037VRW83CF (rhodamine 6G)
RN  - F30N4O6XVV (Acridine Orange)
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Flow Injection Analysis/*methods
MH  - Fluorescence Resonance Energy Transfer/*instrumentation/*methods
MH  - Rhodamines/*chemistry
MH  - Vitamin B 12/*analysis/chemistry
EDAT- 2008/09/23 09:00
MHDA- 2008/11/04 09:00
CRDT- 2008/09/23 09:00
PHST- 2008/01/11 00:00 [received]
PHST- 2008/06/05 00:00 [revised]
PHST- 2008/06/05 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2008/11/04 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
AID - S0039-9140(08)00460-8 [pii]
AID - 10.1016/j.talanta.2008.06.022 [doi]
PST - ppublish
SO  - Talanta. 2008 Oct 19;77(1):176-81. doi: 10.1016/j.talanta.2008.06.022. Epub 2008 
      Jun 25.