PMID- 18804772
OWN - NLM
STAT- MEDLINE
DCOM- 20090112
LR  - 20090115
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1209
IP  - 1-2
DP  - 2008 Oct 31
TI  - Multi-class determination of antimicrobials in meat by pressurized liquid 
      extraction and liquid chromatography-tandem mass spectrometry.
PG  - 162-73
LID - 10.1016/j.chroma.2008.09.011 [doi]
AB  - A multi-residue method using pressurized liquid extraction (PLE) and liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for 
      determining trace levels of 31 antimicrobials, including beta-lactams, 
      lincosamides, macrolides, quinolones, sulfonamides, tetracyclines, 
      nitroimidazoles and trimethoprim. The extraction method required 
      pre-homogeneization of the meat with EDTA-washed sand and subsequent 
      one-static-cycle extraction for 10 min with 40 ml of water at 1500 psi and 70 
      degrees C. The effect of operation temperature, pressure, flush volume, and 
      static cycles on PLE performance was studied. Average recoveries ranged from 75 
      to 99% with relative standard deviations <18%. The method was validated according 
      to the European Union requirements (2002/657/EC). In addition to the quality 
      parameters included in that decision, the limits of detection (LODs) and 
      quantification (LOQs) were determined. The use of LC-MS/MS provided LODs (between 
      3 and 15 microg kg(-1)) and LOQs (between 10 and 50 microg kg(-1)), by far lower 
      than half of their maximum residue limits (MRLs) (between 50 and 1200 microg 
      kg(-1)). Confirmation of the presence of any of the studied compounds was 
      accomplished in 1h after sample receipt. This methodology has been successfully 
      applied to the analysis of cattle and pig tissue samples from local markets and 
      slaughterhouses of the Valencian Community (Spain). The results showed the 
      presence of some antimicrobials at different concentrations. Quinolones and 
      tetracyclines were the antimicrobials most detected in cattle and pig samples, 
      respectively. Sulfonamides were also frequently detected in both types of 
      samples.
FAU - Carretero, Vanesa
AU  - Carretero V
AD  - Laboratori de Bromatologia i Toxicologia, Facultat de Farmacia, Universitat de 
      Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain.
FAU - Blasco, Cristina
AU  - Blasco C
FAU - Pico, Yolanda
AU  - Pico Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080907
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/chemistry/isolation & purification
MH  - Cattle
MH  - Chromatography, Liquid/*methods
MH  - Meat/*analysis
MH  - Molecular Structure
MH  - Reproducibility of Results
MH  - Swine
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2008/09/23 09:00
MHDA- 2009/01/13 09:00
CRDT- 2008/09/23 09:00
PHST- 2008/06/23 00:00 [received]
PHST- 2008/08/31 00:00 [revised]
PHST- 2008/09/04 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2009/01/13 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
AID - S0021-9673(08)01525-2 [pii]
AID - 10.1016/j.chroma.2008.09.011 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Oct 31;1209(1-2):162-73. doi: 10.1016/j.chroma.2008.09.011. 
      Epub 2008 Sep 7.