PMID- 1880539
OWN - NLM
STAT- MEDLINE
DCOM- 19910930
LR  - 20190724
IS  - 0022-510X (Print)
IS  - 0022-510X (Linking)
VI  - 103
IP  - 2
DP  - 1991 Jun
TI  - Presence of serum anti-human T-lymphotropic virus type I (HTLV-I) IgM antibodies 
      means persistent active replication of HTLV-I in HTLV-I-associated myelopathy.
PG  - 203-8
AB  - We investigated serum IgM antibodies against human T-lymphotropic virus type I 
      (HTLV-I) in 29 HTLV-I associated myelopathy (HAM) patients and 34 HTLV-I 
      carriers, using western blot analysis. Anti-HTLV-I IgM was detected in all 6 
      post-transfusional HAM patients and in 19 of 23 (83%) HAM patients with no 
      history of blood transfusion, but in only 4 of 21 (19%) HTLV-I carriers. In HAM 
      patients, HTLV-I proviral DNA integrated into peripheral blood lymphocyte (PBL) 
      was detected by Southern blot analysis in all of the 6 (100%) and 18 of the 23 
      (78%). In contrast, it was detected in only 2 of 25 (8%) HTLV-I carriers. For the 
      serum anti-HTLV-I IgM and HTLV-I provirus in PBL, the differences between the HAM 
      and HTLV-I carriers were statistically significant (P less than 0.01). Our data 
      indicate that the increased HTLV-I proviral DNA in PBL is produced by the 
      persistent active replication of HTLV-I in HAM. Furthermore, Southern blot 
      analysis showed intense bands in HAM patients with histories of blood 
      transfusion, in whom the progression of the disease had been rapid. We conclude 
      that the persistent active replication of HTLV-I is an important factor in the 
      pathogenesis of HAM.
FAU - Nagasato, K
AU  - Nagasato K
AD  - First Department of Internal Medicine, Nagasaki University School of Medicine, 
      Japan.
FAU - Nakamura, T
AU  - Nakamura T
FAU - Shirabe, S
AU  - Shirabe S
FAU - Shibayama, K
AU  - Shibayama K
FAU - Ohishi, K
AU  - Ohishi K
FAU - Ichinose, K
AU  - Ichinose K
FAU - Tsujihata, M
AU  - Tsujihata M
FAU - Nagataki, S
AU  - Nagataki S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neurol Sci
JT  - Journal of the neurological sciences
JID - 0375403
RN  - 0 (DNA, Viral)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Blotting, Southern
MH  - Blotting, Western
MH  - DNA, Viral/analysis
MH  - Female
MH  - HTLV-I Antibodies/*analysis
MH  - Human T-lymphotropic virus 1/genetics/*physiology
MH  - Humans
MH  - Immunoglobulin M/*analysis
MH  - Male
MH  - Middle Aged
MH  - Paraparesis, Tropical Spastic/immunology/*microbiology
MH  - Proviruses/genetics
MH  - *Virus Replication
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - 0022-510X(91)90165-4 [pii]
AID - 10.1016/0022-510x(91)90165-4 [doi]
PST - ppublish
SO  - J Neurol Sci. 1991 Jun;103(2):203-8. doi: 10.1016/0022-510x(91)90165-4.