PMID- 18807221
OWN - NLM
STAT- MEDLINE
DCOM- 20081114
LR  - 20081013
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 153
IP  - 10
DP  - 2008
TI  - Genetic analysis of two porcine reproductive and respiratory syndrome viruses 
      with different virulence isolated in China.
PG  - 1877-84
LID - 10.1007/s00705-008-0207-6 [doi]
AB  - The S1 and SY0608 strains of porcine reproductive and respiratory syndrome virus 
      (PRRSV) were individually isolated and had different pathogenicity in pigs in 
      1997 and 2006. In order to understand their genomic characteristics, the 
      full-length genome of S1 and SY0608 isolates were sequenced and analyzed. The 
      results indicated that their genome composition differed significantly and shared 
      only 88.5% nucleotide identity with each other. The genetic variation and amino 
      acid substitutions were not randomly distributed in the genome, and mainly 
      focused on ORF1a, ORF3 and ORF5. The SY0608 strain, with high pathogenicity, had 
      a 30-amino-acid deletion at amino acid positions 480 and 532-560 in comparison 
      with the S1 strain. The alignment of amino acid sequence of Nsp1-Nsp8, GP2-GP5, M 
      and N of S1 and SY0608 with other PRRSV isolates demonstrated that variation was 
      mainly found in the Nsp2, GP3 and GP5 proteins. In comparison with the S1 strain, 
      the SY0608 strain showed some potential glycosylation site mutations in GP5 at 
      amino acid positions between 26 and 39, which might be associated with viral 
      antigenicity. Phylogenetic analysis showed that the two strains belonged to two 
      different branches that do not indicate differences in pathogenicity. 
      Interestingly, the deletion strains isolated recently in China formed a new minor 
      branch, revealing the same evolutionary trend.
FAU - Li, Yufeng
AU  - Li Y
AD  - Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of 
      Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu, China.
FAU - Wang, Xinglong
AU  - Wang X
FAU - Jiang, Ping
AU  - Jiang P
FAU - Chen, Wen
AU  - Chen W
FAU - Wang, Xianwei
AU  - Wang X
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080922
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Amino Acid Substitution/genetics
MH  - Animals
MH  - China
MH  - Cluster Analysis
MH  - *Genome, Viral
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification/*pathogenicity
MH  - RNA, Viral/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Sequence Homology, Nucleic Acid
MH  - Swine
MH  - Viral Nonstructural Proteins/genetics
MH  - Viral Structural Proteins/genetics
MH  - Virulence
EDAT- 2008/09/23 09:00
MHDA- 2008/11/15 09:00
CRDT- 2008/09/23 09:00
PHST- 2008/04/16 00:00 [received]
PHST- 2008/08/25 00:00 [accepted]
PHST- 2008/09/23 09:00 [pubmed]
PHST- 2008/11/15 09:00 [medline]
PHST- 2008/09/23 09:00 [entrez]
AID - 10.1007/s00705-008-0207-6 [doi]
PST - ppublish
SO  - Arch Virol. 2008;153(10):1877-84. doi: 10.1007/s00705-008-0207-6. Epub 2008 Sep 
      22.