PMID- 1880824
OWN - NLM
STAT- MEDLINE
DCOM- 19910930
LR  - 20211216
IS  - 0022-5304 (Print)
IS  - 0022-5304 (Linking)
VI  - 94
IP  - 4
DP  - 1991 Aug
TI  - Guinea-pig ileal loop assay: a better replacement of the suckling mouse assay for 
      detection of heat-stable enterotoxins of Escherichia coli.
PG  - 234-40
AB  - The suitability of guinea-pig ileal loop assay (GILA) for the assay of 
      heat-stable (ST) enterotoxin was confirmed. Secretory response against 
      Escherichia coli heat-stable enterotoxin in this model was determined in terms of 
      dilatation index (DI). DI equal to 0.50 or more was considered as a positive 
      secretory response. Kinetics of fluid accumulation and the titration of toxin in 
      guinea-pig ileal loop suggest uniform secretory response throughout the small 
      intestine and 31.5 microgram of crude ST toxin as the minimum effective dose to 
      induce a DI of 0.5. Guinea-pig intestine was found sensitive to both methanol 
      soluble (STa) and methanol insoluble (STb) toxins of E. coli and so was 
      considered superior to the existing suckling mouse assay (SMA), which is known to 
      be sensitive only to STa toxin. In addition, GILA was also found to be more 
      suitable and economical as at least 10 strains together with the positive and 
      negative controls can be tested in one animal, whereas in SMA, four suckling mice 
      were needed to test a single strain. Hence, in SMA individual susceptibility 
      among mice cannot be ruled out. GILA was considered to be an alternative to the 
      presently available test, SMA, in the determination of ST toxin of E. coli.
FAU - Choudhry, M A
AU  - Choudhry MA
AD  - Division of Microbiology, Central Drug Research Institute, Lucknow, India.
FAU - Gupta, S
AU  - Gupta S
FAU - Yadava, J N
AU  - Yadava JN
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Trop Med Hyg
JT  - The Journal of tropical medicine and hygiene
JID - 0406044
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Animals
MH  - Animals, Suckling/microbiology
MH  - Bacterial Toxins/*analysis
MH  - *Biological Assay/methods
MH  - Enterotoxins/*analysis
MH  - *Escherichia coli
MH  - *Escherichia coli Proteins
MH  - Guinea Pigs
MH  - Ileum/microbiology
MH  - Mice
MH  - Sensitivity and Specificity
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
PST - ppublish
SO  - J Trop Med Hyg. 1991 Aug;94(4):234-40.