PMID- 18810378
OWN - NLM
STAT- MEDLINE
DCOM- 20090326
LR  - 20181113
IS  - 0946-2716 (Print)
IS  - 0946-2716 (Linking)
VI  - 86
IP  - 12
DP  - 2008 Dec
TI  - Chromosomal changes characterize head and neck cancer with poor prognosis.
PG  - 1353-65
LID - 10.1007/s00109-008-0397-0 [doi]
AB  - It is well established that genetic alterations may be associated to prognosis in 
      tumor patients. This study investigates chromosomal changes that predict the 
      clinical outcome of head and neck squamous cell carcinoma (HNSCC) and correlate 
      to characteristic clinicopathological parameters. We applied comparative genomic 
      hybridization (CGH) to tissue samples from 117 HNSCC patients scheduled for 
      radiotherapy. Genomic aberrations occurring in more than five patients were 
      studied for impact on locoregional progression (LRP)-free survival. p values were 
      adjusted by the Hochberg-Benjamini procedure and significant aberrations and 
      clinical variables subjected to a stepwise backwards Cox proportional model. 
      Significant alterations were further analyzed by array-CGH and fluorescence in 
      situ hybridization (FISH). In multivariate survival analysis gains on 1q and 16q 
      predict reduced LRP-free survival independently from known prognostic factors. 
      Cluster analysis separated the HNSCC cases into two groups (cluster 1 and 2) that 
      are characterized by significant differences for imbalances in 13 chromosomal 
      regions. Moreover, it became apparent that cluster 1 correlates to nonanemic 
      patients, while cluster 2 represents predominantly anemic cases. Array-CGH 
      pinpoints 16q24.3 to be the region of interest on chromosome 16 which was further 
      verified by FISH analysis where an increased copy number of FANCA, a member of 
      the Fanconi anemia/breast cancer pathway, could be identified. This study 
      demonstrates that chromosomal gains on 1q and 16q as well as chromosomal loss on 
      18q represent prognostic markers in HNSCC and that these alterations may explain 
      to some extent the dismal course of a subgroup of patients.
FAU - Bauer, Verena L
AU  - Bauer VL
AD  - Institute of Molecular Radiation Biology, Helmholtz Center Munich German Research 
      Center for Environmental Health GmbH, Ingolstaedter Landstrasse 1, 85764 
      Neuherberg, Germany.
FAU - Braselmann, Herbert
AU  - Braselmann H
FAU - Henke, Michael
AU  - Henke M
FAU - Mattern, Dominik
AU  - Mattern D
FAU - Walch, Axel
AU  - Walch A
FAU - Unger, Kristian
AU  - Unger K
FAU - Baudis, Michael
AU  - Baudis M
FAU - Lassmann, Silke
AU  - Lassmann S
FAU - Huber, Reinhard
AU  - Huber R
FAU - Wienberg, Johannes
AU  - Wienberg J
FAU - Werner, Martin
AU  - Werner M
FAU - Zitzelsberger, Horst F
AU  - Zitzelsberger HF
LA  - eng
PT  - Journal Article
DEP - 20080923
PL  - Germany
TA  - J Mol Med (Berl)
JT  - Journal of molecular medicine (Berlin, Germany)
JID - 9504370
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/*analysis
MH  - Carcinoma, Squamous Cell/diagnosis/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Comparative Genomic Hybridization
MH  - Female
MH  - Head and Neck Neoplasms/diagnosis/*genetics/pathology
MH  - Humans
MH  - Male
MH  - Prognosis
EDAT- 2008/09/24 09:00
MHDA- 2009/03/27 09:00
CRDT- 2008/09/24 09:00
PHST- 2008/03/14 00:00 [received]
PHST- 2008/07/23 00:00 [accepted]
PHST- 2008/07/08 00:00 [revised]
PHST- 2008/09/24 09:00 [pubmed]
PHST- 2009/03/27 09:00 [medline]
PHST- 2008/09/24 09:00 [entrez]
AID - 10.1007/s00109-008-0397-0 [doi]
PST - ppublish
SO  - J Mol Med (Berl). 2008 Dec;86(12):1353-65. doi: 10.1007/s00109-008-0397-0. Epub 
      2008 Sep 23.