PMID- 18814270
OWN - NLM
STAT- MEDLINE
DCOM- 20081027
LR  - 20080929
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Linking)
VI  - 80
IP  - 11
DP  - 2008 Nov
TI  - Human herpesvirus 6 integrates within telomeric regions as evidenced by five 
      different chromosomal sites.
PG  - 1952-8
LID - 10.1002/jmv.21299 [doi]
AB  - Fluorescent in situ hybridization (FISH) was used to investigate the chromosomal 
      integration sites of human herpesvirus 6 (HHV-6) in phytohemagglutinin-stimulated 
      leukocytes and B lymphocytes from Epstein-Barr virus transformed lymphoblastoid 
      cell lines (LCLs). Five different chromosomal integration sites were found in 
      nine individuals. Only one site was identified in each individual, each site was 
      in the vicinity of the telomeric region and was on either the p or q arm of only 
      one of the two chromosome homologues. The sites were 9q34.3, 10q26.3, 11p15.5, 
      17p13.3, and 19q 13.4, of which three have not been previously identified. For 
      9q34.3 the site of integration was further mapped using a locus-specific probe 
      for 9q34.3 together with a pan-telomeric probe and both co-localized with the 
      HHV-6 signal. Similarly an arm-specific telomeric probe for 19q co-localized with 
      the HHV-6 signal. It was therefore concluded that the site of integration is 
      actually within the telomere. The number of viral DNA copies/cell was calculated 
      in blood, LCL cells and hair follicles and was one or more in every case for each 
      of the nine individuals. This result was confirmed by FISH where 100% of cells 
      gave an HHV-6 signal. These findings add to previous reports suggesting that 
      integrated HHV-6 DNA is found in every cell in the body and transmitted 
      vertically. Finally, including our data, worldwide seven different chromosomal 
      sites of HHV-6 integration have now been identified. Large epidemiological 
      studies of chromosomal integration are required to identify further telomeric 
      sites, geographical or racial variation and possible clinical consequences.
CI  - 2008 Wiley-Liss, Inc.
FAU - Nacheva, Elisabeth P
AU  - Nacheva EP
AD  - Department of Haematology, Royal Free & University College Medical School 
      (Hampstead Campus), London, United Kingdom. e.nacheva@ucl.ac.uk
FAU - Ward, Katherine N
AU  - Ward KN
FAU - Brazma, Diana
AU  - Brazma D
FAU - Virgili, Anna
AU  - Virgili A
FAU - Howard, Julie
AU  - Howard J
FAU - Leong, Hoe Nam
AU  - Leong HN
FAU - Clark, Duncan A
AU  - Clark DA
LA  - eng
GR  - 051350/Z/Wellcome Trust/United Kingdom
GR  - 059040/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Chromosomes, Human/virology
MH  - Female
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - Leukocytes/virology
MH  - Male
MH  - Middle Aged
MH  - Telomere/*virology
MH  - *Virus Integration
EDAT- 2008/09/25 09:00
MHDA- 2008/10/28 09:00
CRDT- 2008/09/25 09:00
PHST- 2008/09/25 09:00 [pubmed]
PHST- 2008/10/28 09:00 [medline]
PHST- 2008/09/25 09:00 [entrez]
AID - 10.1002/jmv.21299 [doi]
PST - ppublish
SO  - J Med Virol. 2008 Nov;80(11):1952-8. doi: 10.1002/jmv.21299.