PMID- 18814932
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20210208
IS  - 0169-5002 (Print)
IS  - 0169-5002 (Linking)
VI  - 64
IP  - 1
DP  - 2009 Apr
TI  - Chromosomal instability is a risk factor for poor prognosis of adenocarcinoma of 
      the lung: Fluorescence in situ hybridization analysis of paraffin-embedded tissue 
      from Korean patients.
PG  - 66-70
LID - 10.1016/j.lungcan.2008.07.016 [doi]
AB  - BACKGROUND: In this study, we sought to evaluate the prognostic importance of 
      chromosomal instability (CIN) in adenocarcinoma (AC) of the lung. The 
      relationship between CIN detected by fluorescence in situ hybridization (FISH) 
      and survival in AC patients was examined. METHODS: Sixty-three surgical specimens 
      of lung AC were analyzed. To identify tumors with CIN, p16 and multi-target DNA 
      FISH assays for c-myc, chromosome 6, EGFR, and chromosome 5 (LAVysion, Vysis) 
      were performed on nuclei extracted from paraffin-embedded tumor tissues. Survival 
      rates were compared in terms of sex, age, histology, T factor, N factor, CIN, and 
      smoking status. A sample was classified as CIN-positive if at least three of the 
      five chromosomes were positive. RESULTS: Out of the 63 specimens, 32 (39.7%) were 
      CIN-positive. The 5-year overall disease-free survival rate was 58.7% as a whole, 
      46.9% for CIN-positive patients and 71.0% for the CIN-negative patients [hazard 
      ratio (HR), 2.34; 95% confidence interval (CI), 1.04-5.26; p = 0.04]. The 5-year 
      overall survival rate was 81.0%, 68.7% for CIN-positive patients and 93.5% for 
      the CIN-negative patients (HR, 5.64; 95% CI, 1.23-25.70; p = 0.026). In 
      multivariate analysis after adjusting for pathologic nodal staging, tumor 
      staging, sex, age, and smoking history, compared with the CIN-negative patients, 
      the CIN-positive status remained significantly associated with decreased overall 
      survival (HR, 8.48; 95% CI, 1.66-43.42; p = 0.010). CONCLUSIONS: CIN can be 
      effectively detected in primary AC of lung using FISH analysis. CIN is associated 
      with poor prognosis for AC, and may thus be utilized as an independent prognostic 
      factor for the disease.
FAU - Choi, Chang-Min
AU  - Choi CM
AD  - Department of Internal Medicine, Asan Medical Center, College of Medicine, 
      University of Ulsan, Seoul, South Korea.
FAU - Seo, Kwang Won
AU  - Seo KW
FAU - Jang, Se Jin
AU  - Jang SJ
FAU - Oh, Yeon-Mok
AU  - Oh YM
FAU - Shim, Tae-Sun
AU  - Shim TS
FAU - Kim, Woo Sung
AU  - Kim WS
FAU - Lee, Dong-Soon
AU  - Lee DS
FAU - Lee, Sang-Do
AU  - Lee SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080923
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
RN  - 0 (CDKN2A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (MYC protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Adult
MH  - Aged
MH  - *Chromosomal Instability
MH  - Chromosomes, Human, Pair 6/*genetics
MH  - Cyclin-Dependent Kinase Inhibitor p16
MH  - ErbB Receptors/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Korea
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*genetics
MH  - Paraffin Embedding
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-myc/*genetics
MH  - Risk Factors
MH  - Survival Rate
EDAT- 2008/09/26 09:00
MHDA- 2009/05/08 09:00
CRDT- 2008/09/26 09:00
PHST- 2008/06/10 00:00 [received]
PHST- 2008/07/24 00:00 [revised]
PHST- 2008/07/27 00:00 [accepted]
PHST- 2008/09/26 09:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
PHST- 2008/09/26 09:00 [entrez]
AID - S0169-5002(08)00414-5 [pii]
AID - 10.1016/j.lungcan.2008.07.016 [doi]
PST - ppublish
SO  - Lung Cancer. 2009 Apr;64(1):66-70. doi: 10.1016/j.lungcan.2008.07.016. Epub 2008 
      Sep 23.